Age of callus tissues and cotyledonary materials on the selection of cocoa swollen shoot virus-free somatic embryos

Authors

  • A. K. Quainoo Department of Biotechnology, University for Development Studies, Tamale, Ghana

Keywords:

cocoa swollen shoot virus (CSSV), primary embryogenesis, secondary embryogenesis, somatic embryos, PCR/capillary electrophoresis, PCR/agalose electrophoresis

Abstract

Research was conducted to investigate the effect of age of callus tissue and cotyledonary material on the selection of CSSV-free cocoa somatic embryos. Polymerase chain reaction (PCR) capillary electrophoresis was more sensitive and quick in detecting the CSSV than PCR/agarose electrophoresis. PCR/capillary electrophoresis revealed the presence of CSSV in callus tissues in 1wk at the rate of 53(82 %), 47(94 %) and 46(85 %) for three infected Amelonado cocoa trees, T1, T2 and T4, respectively while  PCR/agarose electrophoresis recorded 23(36 %), 19(38 %) and 26(48 %) for T1, T2 and T4), respectively.

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Published

15-04-2011

How to Cite

Quainoo, A. K. (2011). Age of callus tissues and cotyledonary materials on the selection of cocoa swollen shoot virus-free somatic embryos. Research in Biotechnology, 2(2). Retrieved from https://updatepublishing.com/journal/index.php/rib/article/view/2339

Issue

Vol. 2 No. 2 (2011)

Section

Research Articles