<em>In vitro</em> micropropagation of <em>Alpinia zerumbet</em> Variegate, an important medicinal plant, through rhizome bud explants

Abstract

An ideal Micropropagation method of Alpinia zerumbet has been developed using rhizome bud explants. Basal MS medium supplemented with 3% sucrose (w/v) different concentrations of BAP in combination with 0.5 mg / L of kinetin. Highest percentage (95%) of explants for shoot induction and multiple shoot (7.9 per explants) production were observed in the  combination of 1.5 mg / L of BAP, 0.5 mg / L of kinetin. In this case, all the inoculated explants induced multiple shoots within 6-7 weeks of inoculation. Rooting was induced in a medium having half strength MS supplemented with 0.5mg / L of IBA. Most of the generated shoots were successfully transferred to soil under field conditions.