In vitro assessment of Trichoderma asperellum interaction with combination fungicides

Authors

  • Tejashri T. Khore Department of Plant Pathology, Dr. Sharadchandra Pawar College of Agriculture, Baramati, Pune-413115, Maharashtra, India
  • Chandrashekhar V. Shende Department of Plant Pathology, Dr. Sharadchandra Pawar College of Agriculture, Baramati, Pune-413115, Maharashtra, India
  • Shital S. Dhawan Department of Plant Pathology, Dr. Sharadchandra Pawar College of Agriculture, Baramati, Pune-413115, Maharashtra, India

DOI:

https://doi.org/10.25081/jsa.2026.v10.9810

Keywords:

Trichoderma asperellum, Biological control, Compatibility, Fungicides, Integrated disease management

Abstract

This study was carried out to assess how well Trichoderma asperellum, a widely used biological control agent, performs in the presence of selected combination group fungicides. The experiment took place during 2024-2025 at the Department of Plant Pathology, Dr. Sharadchandra Pawar College of Agriculture, Baramati, using a Completely Randomized Design (CRD). Seven combination fungicides— Ridomil Gold (Metalaxyl M 4% + Mancozeb 64% WP), Folio Gold (Metalaxyl M 33% + Chlorothalonil 33.1% SC), Indofil Sprint (Carbendazim 25% + Mancozeb 50% WS), Amistar Top (Azoxystrobin 18.2% + Difenoconazole 11.4% w/w SC), Syngenta Gloit (Propiconazole 13.9% + Difenoconazole 13.9% EC), Dhanuka Lustre (Carbendazim 25% + Flusilazole 12.5% SE), Bayer Nativo (Tebuconazole 50% +Trifloxystrobin 25% WG) were tested at both full and half of their recommended doses using the poison food technique. Results showed that Metalaxyl M 4% + Mancozeb 64% WP was highly compatible with 22.22 per cent growth inhibition at half concentration and moderate compatibility with 53.70 per cent growth inhibition at recommended concentration with T. asperellum, while the remaining fungicides significantly suppressed the growth of the biocontrol agent with 83.52 to 94.44 per cent growth inhibition. These findings highlight the potential for combining T. asperellum with compatible fungicides like Metalaxyl M 4% + Mancozeb 64% WP in integrated disease management programs, while suggesting caution or avoidance for those with inhibitory effects.

Downloads

Download data is not yet available.

References

Bai, A. T., Vibha, J. B., Nair, R., & Upadhyay, A. (2022). Compatibility of Trichoderma viride with commonly used fungicides in management of early blight pathogen of tomato. The Pharma Innovation Journal, 11(1), 1734-1736.

Barnett, H. L., & Binder, F. L. (1973). The fungal host-parasite relationship. Annual Review of Phytopathology, 11, 273-292. https://doi.org/10.1146/annurev.py.11.090173.001421

Bharadwaz, P., Nath, B. C., Chetia, R., Saikia, S., Bora, P., & Bhattacharyya, P. N. (2023). In vitro studies on the compatibility of Trichoderma viride with commonly used agrochemicals in the vegetable cropping system. Pest Management in Horticultural Ecosystems, 29(1), 136-143.

Bheemaraya, Patil, M., Ramesh, Vendan, K. T., Amaresh, Y. S., & Rao, K. (2012). Compatibility of Trichoderma spp. With commonly used fungicides, insecticides and plant extracts. Indian Journal of Plant Protection, 40(2), 118-122.

Chu, F. F., & Wu, W. S. (1981). Biological and chemical control of Rhizoctonia solani by pea seed treatment. National Taiwan University, 21, 19-28.

Cook, R. I., & Baker, K. F. (1983). The nature and practices of biological control of plant pathogens. APS Books.

Elad, Y., Chet, I., Boyle, P., & Henis, Y. (1983). Parasitism of Trichoderma spp. on Rhizoctonia solani and Sclerotium rolfsii-scanning electron microscopy and fluorescence microscopy. Phytopathology, 73(1), 85-88.

Harman, G. E., & Kubicek, C. P. (1998). Trichoderma and gliocladium in biological control: an overview. In G. E. Harman & C. P. Kubicek (Eds.), Trichoderma and Gliocladium: Enzymes, biological control and commercial applications (Vol. 2, pp. 131-151). CRC Press.

Harman, G. E., & Kubicek, C. P. (2002). Trichoderma and Gliocladium: Basic biology, taxonomy and genetics. (1st ed.). CRC Press. https://doi.org/10.1201/9781482295320

Harman, G. E., Latorre, B., Agosin, E., San Martin, R., Riegel, D. G., Nielsen, P. A., Tronsmo, A., & Pearson, R. C. (1996). Biological and Integrated Control of Botrytis Bunch Rot of Grape Using Trichoderma spp. Biological Control, 7(3), 259-266. https://doi.org/10.1006/bcon.1996.0092

Johnson, L. F., & Curl, E. A. (1972). Methods for research on the ecology of soil-borne plant pathogens. Burgess Publishing Company.

Kumar, R., Singh, S. K., Yadav, S., Kumar, R., Choubey, A. K., & Kumari, A. (2018). Compatibility of Trichoderma viride with different fungicide and organic cake. Journal of Pharmacognosy and Phytochemistry, 7(2), 2398-2401.

Kumar, S., Kumar, A., Kumar, R., & Rajesha, G. (2021). Study on compatibility of Trichoderma viride with different fungicides. The Indian Journal of Agricultural Sciences, 91(12), 1788-1792. https://doi.org/10.56093/ijas.v91i12.120808

Kumar, T. V., Veena, S. S., Karthikeyan, S., & Sreekumar, J. (2017). Compatibility of Trichoderma asperellum with fungicides, insecticides, inorganic fertilizers and bio-pesticides. Journal of Root Crops, 43(2), 68-75.

Maheshwary, N. P., Gangadhara Naik, B., Chittaragi, A., Naik, S. K., Satish, K. M., & Nandish, M. S. (2020). Compatibility of Trichoderma asperellum with fungicides. The Pharma Innovation Journal, 9(8), 136-140.

Manandhar, S., Timila, R. D., Karkee, A., Gupt, S. K., & Baidya, S. (2020). Compatibility study of Trichoderma isolates with chemical fungicides. Journal of Agriculture and Environment, 21, 9-18.

Nene, Y. L., & Thapliyal, P. N. (1993). Fungicides in plant disease control. (3rd ed.). IBH Publishing Company.

Panse, V. G., & Sukhatme, P. V. (1967). Statistical analysis for agricultural workers. ICAR.

Poudel, S., Pun, L. B., Paudel, R., Neupane, S., Pokharel, S., & Khanal, P. (2024). Compatibility study of Trichoderma sp. with Chemical Fungicides Commonly Used by Nepalese Farmers, Under In-Vitro Condition. International Journal of Applied Biology, 8(1), 51-61.

Saha, S., Pharate, S., Thosar, R. U., & Chavan, V. (2023). Compatibility of Trichoderma asperelloides with Fungicides Controlling Downy Mildew and Powdery Mildew in Grapes. Grape Insight, 1(1), 32-36.

Veena, S. S., Baby, A., & Karthikeyan, S. (2022). Study on compatibility of Trichoderma asperellum and fungicides for the development of environment friendly and cost-effective disease management strategies. Journal of Root Crops, 48(1 & 2), 35-40.

Vincent, J. M. (1927). Distoration of fungal hyphae in the presence of certain inhibitors. Nature, 159, 180. https://doi.org/10.1038/159850b0

Yao, X., Guo, H., Zhang, K., Zhao, M., Ruan, J., & Chen, J. (2023). Trichoderma and its role in biological control of plant fungal and nematode disease. Frontiers in Microbiology, 14, 1160551. https://doi.org/10.3389/fmicb.2023.1160551

Published

18-02-2026

How to Cite

Khore, T. T., Shende, C. V., & Dhawan, S. S. (2026). In vitro assessment of Trichoderma asperellum interaction with combination fungicides. Journal of Scientific Agriculture, 10, 13–16. https://doi.org/10.25081/jsa.2026.v10.9810

Issue

Section

Articles