Evaluating the phosphate solubilizing ability of novel Bacillus sp. strains and their effects on corn production
DOI:
https://doi.org/10.25081/jsa.2026.v10.9784Keywords:
Phosphate solubilizing bacteria, Bacillus sp., Fertilizer management, Corn production, Soil propertiesAbstract
This study investigated the effects of two phosphate solubilizing bacteria (PSB) isolates, Bacillus sp. strain E1 and G4, and three fertilizer types on soil properties, corn growth, and yield. The results showed that PSB E1 increased soil organic matter (SOM) and available phosphorus, while G4 enhanced exchangeable potassium and cation exchange capacity (CEC). Organic fertilizers improved soil fertility, while inorganic fertilizers had a different impact. The combination of PSB G4 and organic fertilizer produced the highest yield, while PSB E1 combined with fertilizers boosted crop growth. The interaction between PSB strains and fertilizers played a significant role in determining soil properties, corn growth, and yield.
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Abrol, V., Sharma, P., Chary, G. R., Srinivasarao, C., Sankar, G. R. M., Singh, B., Kumar, A., Hashem, A., Ibrahimova, U., Abd-Allah, E. F., & Kumar, M. (2024). Integrated organic and mineral fertilizer strategies for achieving sustainable maize yield and soil quality in dry sub-humid inceptisols. Scientific Reports, 14, 27227. https://doi.org/10.1038/s41598-024-74727-4
Adeleke, R., Nwangburuka, C., & Oboirien, B. (2017). Origins, roles and fate of organic acids in soils: A review. South African Journal of Botany, 108, 393-406. https://doi.org/10.1016/j.sajb.2016.09.002
Adesemoye, A. O., & Kloepper, J. W. (2009). Plant-microbes interactions in enhanced fertilizer-use efficiency. Applied Microbiology and Biotechnology, 85, 1-12. https://doi.org/10.1007/s00253-009-2196-0
Adnan, M., Shah, Z., Fahad, S., Arif, M., Alam, M., Khan, I. A., Mian, I. A., Basir, A., Ullah, H., Arshad, M., Rahman, I.-U., Saud, S., Ihsan, M. Z., Jamal, Y., Amanullah, Hammad, H. M., & Nasim, W. (2017). Phosphate-solubilizing bacteria nullify the antagonistic effect of soil calcification on bioavailability of phosphorus in alkaline soils. Scientific Reports, 7, 16131. https://doi.org/10.1038/s41598-017-16537-5
Al-Maamori, H. A., Salman, A. D., Al-Budeiri, M., Alshami, Y. A. O., & Al-Shaabani, E. M. (2018). Effect of vermicompost production on some soil properties and nutrients in plants. IOP Conference Series: Earth and Environmental Science, 1214, 012006. https://doi.org/10.1088/1755-1315/1214/1/012006
Alori, E. T., Glick, B. R., & Babalola, O. O. (2017). Microbial phosphorus solubilization and its potential for use in sustainable agriculture. Frontiers in Microbiology, 8, 971. https://doi.org/10.3389/fmicb.2017.00971
Assefa, S., & Tadesse, S. (2019). The principal role of organic fertilizer on soil properties and agricultural productivity - A review. Agricultural Research and Technology, 22(2), 556192. https://doi.org/10.19080/ARTOAJ.2019.22.556192
Atiyeh, R. M., Lee, S., Edwards, C. A., Arancon, N. Q., & Metzger, J. D. (2002). The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Bioresource Technology, 84(1), 7-14. https://doi.org/10.1016/S0960-8524(02)00017-2
Bender, S. F., Wagg, C., & van der Heijden, M. G. A. (2016). An underground revolution: biodiversity and soil ecological engineering for agricultural sustainability. Trends in Ecology & Evolution, 31(6), 440-452. https://doi.org/10.1016/j.tree.2016.02.016
Bhattacharyya, P. N., & Jha, D. K. (2012). Plant growth-promoting rhizobacteria (PGPR): Emergence in agriculture. World Journal of Microbiology and Biotechnology, 28, 1327-1350. https://doi.org/10.1007/s11274-011-0979-9
Bo, H., Li, Z., Wang, W., Zhang, R., Wang, H., Jin, D., Xu, M., & Zhang, Q. (2024). Combining organic and inorganic fertilization enhances soil enzyme activity, the bacterial community, and molecular ecological network complexity in coal mine reclamation areas. Agronomy, 14(7), 1427. https://doi.org/10.3390/agronomy14071427
Brady, N. C., & Weil, R. R. (2008). The nature and properties of soils. (14th ed.). Pearson Prentice Hall.
Brady, N. C., & Weil, R. R. (2017). The nature and properties of soils. (15th ed.). Pearson Prentice Hall.
BSWM. (2022). Guidelines for the interpretation of general soil fertility status. Bureau of Soil and Water Management.
Chen, S., Gao, J., Chen, H., Zhang, Z., Huang, J., Lv, L., Tan, J., & Jiang, X. (2023). The role of long-term mineral and manure fertilization on P species accumulation and phosphate-solubilizing microorganisms in paddy red soils. Soil, 9, 101-116. https://doi.org/10.5194/soil-9-101-2023
Compant, S., Clément, C., & Sessitsch, A. (2010). Plant growth-promoting bacteria in the rhizo- and endosphere of plants: Their role, colonization, mechanisms involved and prospects for utilization. Soil Biology and Biochemistry, 42(5), 669-678. https://doi.org/10.1016/j.soilbio.2009.11.024
da Silva, L. I., Pereira, M. C., de Carvalho, A. M. X., Buttros, V. H., Pasqual, M., & Doria, J. (2023). Phosphorus-solubilizing microorganisms: A key to sustainable agriculture. Agriculture, 13(2), 462. https://doi.org/10.3390/agriculture13020462
Darzi, M. T., Seyedhadi, M. H., & Rejali, F. (2012). Effects of the application of vermicompost and phosphate solubilizing bacterium on the morphological traits and seed yield of anise (Pimpinella anisum L.). Journal of Medicinal Plants Research, 6(2), 215-219. https://doi.org/10.5897/JMPR2025.7411
de Abreu, C. S., Figueiredo, J. E. F., Oliveira, C. A., Dos Santos, V. L., Gomes, E. A., Ribeiro, V. P., Barros, B. A., Lana, U. G. P., & Marriel, I. E. (2017). Maize endophytic bacteria as mineral phosphate solubilizers. Genetics and Molecular Research, 16(1), gmr16019294.
Diacono, M., & Montemurro, F. (2010). Long-term effects of organic amendments on soil fertility. Agronomy for Sustainable Development, 30, 401-422. https://doi.org/10.1051/agro/2009040
Elhaissoufi, W., Ghoulam, C., Barakat, A., Zeroual, Y., & Bargaz, A. (2022). Phosphate bacterial solubilization: A key rhizosphere driving force enabling higher P use efficiency and crop productivity. Journal of Advanced Research, 38, 13-28. https://doi.org/10.1016/j.jare.2021.08.014
Fontaine, S., Barot, S., Barré, P., Bdioui, N., Mary, B., & Rumpel, C. (2007). Stability of organic carbon in deep soil layers controlled by fresh carbon supply. Nature, 450, 277-280. https://doi.org/10.1038/nature06275
Gaur, A. C. (1990). Phosphate solubilizing microorganisms as biofertilizers. Omega Scientific Publishers.
Geisseler, D., & Scow, K. M. (2014). Long-term effects of mineral fertilizers on soil microorganisms - A review. Soil Biology and Biochemistry, 75, 54-63. https://doi.org/10.1016/j.soilbio.2014.03.023
Glick, B. R. (2012). Plant growth-promoting bacteria: Mechanisms and applications. Scientifica, 2012, 963401. https://doi.org/10.6064/2012/963401
Goswami, D., Thakker, J. N., & Dhandhukia, P. C. (2017). Portraying mechanics of plant growth promoting rhizobacteria (PGPR): A review. Cogen Food & Agriculture, 2(1), 1127500. https://doi.org/10.1080/23311932.2015.1127500
Han, H. S., Supanjani, & Lee, K. D. (2006). Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant, Soil and Environment, 52(3), 130-137. https://doi.org/10.17221/3356-PSE
Han, P., Zhang, W., Wang, G., Sun, W., & Huang, Y. (2016). Changes in soil organic carbon in croplands subjected to fertilizer management: a global meta-analysis. Scientific Reports, 6, 27199. https://doi.org/10.1038/srep27199
Havlin, J. L., Tisdale, S. L., Nelson, W. L., & Beaton, J. D. (2014). Soil fertility and fertilizers: An introduction to nutrient management. (8th ed.). Pearson Education.
Haynes, R. J., & Naidu, R. (1998). Influence of lime, fertilizer and manure applications on soil organic matter content and soil physical conditions: A review. Nutrient Cycling in Agroecosystems, 51, 123-137. https://doi.org/10.1023/A:1009738307837
Illmer, P., & Schinner, F. (1995). Solubilization of inorganic calcium phosphates—Solubilization mechanisms. Soil Biology and Biochemistry, 27(3), 257-263. https://doi.org/10.1016/0038-0717(94)00190-C
Islam, M. A., Ferdous, G., Akter, A., Hossain, M. M., & Nandwani, D. (2017). Effect of organic, inorganic fertilizers and plant spacing on the growth and yield of cabbage. Agriculture, 7(4), 31. https://doi.org/10.3390/agriculture7040031
Kalayu, G. (2019). Phosphate solubilizing microorganisms: Promising approach as biofertilizers. International Journal of Agronomy, 2019, 4917256. https://doi.org/10.1155/2019/4917256
Kaur, G., & Reddy, M. S. (2014). Role of phosphate-solubilizing bacteria in improving the soil fertility and crop productivity in organic farming. Archives of Agronomy and Soil Science, 60(4), 549-564. https://doi.org/10.1080/03650340.2013.817667
Khan, M. S., Zaidi, A., & Musarrat, J. (2014). Phosphate solubilizing microorganisms: Principles and application of microphos technology. Springer. https://doi.org/10.1007/978-3-319-08216-5
Khan, M. S., Zaidi, A., & Wani, P. A. (2007). Role of phosphate-solubilizing microorganisms in sustainable agriculture – A review. Agronomy for Sustainable Development, 27, 29-43. https://doi.org/10.1051/agro:2006011
Kuzyakov, Y., Friedel, J. K., & Stahr, K. (2000). Review of mechanisms and quantification of priming effects. Soil Biology and Biochemistry, 32(11-12), 1485-1498. https://doi.org/10.1016/S0038-0717(00)00084-5
Ladha, J., Reddy, C. K., Padre, A. T., & Kessel, C. V. (2011). Role of nitrogen fertilization in sustaining organic matter in cultivated soils. Journal of Environmental Quality, 40(6), 1756-1766. https://doi.org/10.2134/jeq2011.0064
Lal, R. (2005). Soil erosion and carbon dynamics. Soil and Tillage Research, 81(2), 137-142. https://doi.org/10.1016/j.still.2004.09.002
Lazcano, C., Gómez-Brandón, M., & Domínguez, J. (2008). Comparison of the effectiveness of composting and vermicomposting for the biological stabilization of cattle manure. Chemosphere, 72(7), 1013-1019. https://doi.org/10.1016/j.chemosphere.2008.04.016
Li, H., Hu, Z., Wan, Q., Mu, B., Li, G., & Yang, Y. (2022). Integrated application of inorganic and organic fertilizer enhances soil organo-mineral associations and nutrients in tea garden soil. Agronomy, 12(6), 1330. https://doi.org/10.3390/agronomy12061330
Liu, Y., Lan, X., Hou, H., Ji, J., Liu, X., & Lv, Z. (2024). Multifaceted ability of organic fertilizers to improve crop productivity and abiotic stress tolerance: Review and perspective. Agronomy, 14(6), 1141. https://doi.org/10.3390/agronomy14061141
Liu, Y., Nessa, A., Zheng, Q., Hu, D., Zhang, W., & Zhang, M. (2023). Inoculations of phosphate-solubilizing bacteria alter soil microbial community and improve phosphorus bioavailability for moso bamboo (Phyllostachys edulis) growth. Applied Soil Ecology, 189, 104911. https://doi.org/10.1016/j.apsoil.2023.104911
Lodi, L. A., Klaic, R., Bortoletto-Santos, R., Ribeiro, C., & Farinas, C. S. (2022). Unveiling the solubilization of potassium mineral rocks in organic acids for application as K-fertilizer. Applied Biochemistry and Biotechnology, 194, 2431-2447. https://doi.org/10.1007/s12010-022-03826-7
Maeder, P., Fliessbach, A., Dubois, D., Gunst, L., Fried, P., & Niggli, U. (2002). Soil fertility and biodiversity in organic farming. Science, 296(5573), 1694-1697. https://doi.org/10.1126/science.1071148
Mahmud, K., Missaoui, A., Lee, K., Ghimire, B., Presley, H. W., & Makaju, S. (2021). Rhizosphere microbiome manipulation for sustainable crop production. Current Plant Biology, 27, 100210. https://doi.org/10.1016/j.cpb.2021.100210
Meena, V. S., Maurya, B. R., Verma, J. P., & Aeron, A. (2015). Potassium solubilizing microorganisms for sustainable agriculture. Springer. https://doi.org/10.1007/978-81-322-2776-2
Nambu, K., Kunimatsu, T., & Kyuma, K. (1994). Rates of soil acidification under different patterns of nitrogen mineralization. Soil Science and Plant Nutrition, 40(1), 95-106. https://doi.org/10.1080/00380768.1994.10414282
Nath, D., Maurya, B. R., & Meena, V. S. (2017). Documentation of five potassium- and phosphorus-solubilizing bacteria for their K and P-solubilization ability from various minerals. Biocatalysis and Agricultural Biotechnology, 10, 174-181. https://doi.org/10.1016/j.bcab.2017.03.007
Nobile, C. M., Bravin, M. N., Becquer, T., & Paillat, J.-M. (2019). Phosphorus sorption and availability in an andosol after a decade of organic or mineral fertilizer applications: Importance of pH and organic carbon modifications in soil as compared to phosphorus accumulation. Chemosphere, 239, 124709. https://doi.org/10.1016/j.chemosphere.2019.124709
Oehl, F., Frossard, E., Fliessbach, A., Dubois, P., & Oberson, A. (2004). Basal organic phosphorus mineralization in soils under different farming systems. Soil Biology and Biochemistry, 36(4), 667-675. https://doi.org/10.1016/j.soilbio.2003.12.010
Przemieniecki, S. W., Kurowski, T. P., Kotlarz, K., & Mastalerz, J. (2019). The ability of Pseudomonas sp. SP0113 to solubilize tricalcium phosphate and its influence on the development of spring wheat. Polish Journal of Environmental Studies, 28(5), 3533-3538. https://doi.org/10.15244/pjoes/92525
Rafique, M., Sultan, T., Ortas, I., & Chaudhary, H. J. (2017). Enhancement of maize plant growth with inoculation of phosphate-solubilizing bacteria and biochar amendment in soil. Soil Science and Plant Nutrition, 63(5), 460-469. https://doi.org/10.1080/00380768.2017.1373599
Ranjan, A., Mahalakshmi, M. R., & Sridevi, M. (2013). Isolation and characterization of phosphate-solubilizing bacterial species from different crop fields of Salem, Tamil Nadu, India. International Journal of Nutrition Pharmacology Neurological Diseases, 3(1), 29-33.
Ray, D. K., Mueller, N. D., West, P. C., & Foley, J. A. (2013). Yield trends are insufficient to double global crop production by 2050. Plos One, 8(6), e66428. https://doi.org/10.1371/journal.pone.0066428
Richardson, A. E. (2001). Prospect for using soil microorganisms to improve the acquisition of phosphorus by plants. Functional Plant Biology, 28(9), 897-906. https://doi.org/10.1071/PP01093
Richardson, A. E., Barea, J.-M., McNeill, A. M., & Prigent-Combaret, C. (2009). Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and Soil, 321(1-2), 305-339. https://doi.org/10.1007/s11104-009-9895-2
Rodrı́guez, H., & Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances, 17(4-5), 319-339. https://doi.org/10.1016/S0734-9750(99)00014-2
Rodrı́guez, H., Fraga, R., Gonzalez, T., & Bashan, Y. (2006). Genetics of phosphate solubilization and its potential applications for improving plant growth-promoting bacteria. Plant and Soil, 287, 15-21. https://doi.org/10.1007/s11104-006-9056-9
Sawan, Z. M. (2018). Mineral fertilizers and plant growth retardants: Its effects on cottonseed yield; its quality and contents. Cogent Biology, 4(1), 1459010. https://doi.org/10.1080/23312025.2018.1459010
Sharma, S. B., Sayyed, R. Z., Trivedi, M. H., & Gobi, T. A. (2013). Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus, 2, 587. https://doi.org/10.1186/2193-1801-2-587
Singh, B., & Satyanarayana, T. (2011). Microbial phytases in phosphorus acquisition and plant growth promotion. Physiology and Molecular Biology of Plants, 17, 93-103. https://doi.org/10.1007/s12298-011-0062-x
Song, H., Yang, B., Liang, Y., Yang, L., Song, J., & Li, T. (2024). Combined application of balanced chemical and organic fertilizers on improving crop yield by affecting soil macroaggregation and carbon sequestration. Agronomy, 14(12), 2813. https://doi.org/10.3390/agronomy14122813
Tahir, M., Khalid, U., Ijaz, M., Shan, G. M., Naeem, M. A., Shahid, M., Mahmood, K., Ahmad, N., & Kareem, F. (2018). Combined application of bio-organic phosphate and phosphorus solubilizing bacteria (Bacillus strain MWT 14) improve the performance of bread wheat with low fertilizer input under an arid climate. Brazilian Journal of Microbiology, 49(S1), 15-24. https://doi.org/10.1016/j.bjm.2017.11.005
Tan, K. H. (2010). Principles of Soil Chemistry. (4th ed). CRC Press.
Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255, 571-586. https://doi.org/10.1023/A:1026037216893
Vurukonda, S. S. K. P., Vardharajula, S., Shrivastava, M., & SkZ, A. (2016). Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research, 184, 13-24. https://doi.org/10.1016/j.micres.2015.12.003
Walpola, B. C., & Yoon, M.-H. (2013). Phosphate solubilizing bacteria: Assessment of their effect on growth promotion and phosphorus uptake of mung bean (Vigna radiata [L.] R. Wilczek). Chilean Journal of Agricultural Research, 73(3), 275-281. https://doi.org/10.4067/S0718-58392013000300010
Wan, L.-J., Tian, Y., He, M., Zheng, Y.-Q., Lyu, Q., Xie, R.-J., Ma, Y., Deng, L., & Yi, S.-L. (2021). Effects of chemical fertilizer combined with organic fertilizer application on soil properties, citrus growth physiology, and yield. Agriculture, 11(12), 1207. https://doi.org/10.3390/agriculture11121207
Wang, J. L., Liu, K. L., Zhao, X. Q., Zhang, H. Q., Li, D., Li, J. J., & Shen, R. F. (2021). Balanced fertilization over four decades has sustained soil microbial communities and improved soil fertility and rice productivity in red paddy soil. Science of The Total Environment, 793, 148664. https://doi.org/10.1016/j.scitotenv.2021.148664
Wang, J., Liu, G., Cui, N., Liu, E., Zhang, Y., Liu, D., Ren, X., Jia, Z., & Zhang, P. (2023). Suitable fertilization can improve maize growth and nutrient utilization in ridge-furrow rainfall harvesting cropland in semiarid area. Frontiers in Plant Science, 14, 1198366. https://doi.org/10.3389/fpls.2023.1198366
Wang, J., Qin, H., Zhang, L., Tang, Y., Long, J., Xu, H., & Zhu, B. (2024). Synergistic effects of rhizosphere effect and combined organic and chemical fertilizers application on soil bacterial diversity and community structure in oilseed rape cultivation. Frontiers in Microbiology, 15, 1374199. https://doi.org/10.3389/fmicb.2024.1374199
Wu, F., Li, J., Chen, Y., Zhang, L., Zhang, Y., Wang, S., Shi, X., Li, L., & Liang, J. (2019). Effects of phosphate solubilizing bacteria on the growth, photosynthesis, and nutrient uptake of Camellia oleifera Abel. Forests, 10(4), 348. https://doi.org/10.3390/f10040348
Xing, Y., Li, Y., Zhang, F., & Wang, X. (2024). Appropriate application of organic fertilizer can effectively improve soil environment and increase maize yield in loess plateau. Agronomy, 14(5), 993. https://doi.org/10.3390/agronomy14050993
Zhang, C., Zhang, L., Cao, Y., Zhang, S., Hou, C., & Zhang, C. (2024) Effects of microbial organic fertilizer, microbial inoculant, and quicklime on soil microbial community composition in pepper (Capsicum annuum L.) continuous cropping system. Horticulturae, 10(11), 1142. https://doi.org/10.3390/horticulturae10111142
Zhang, C., Zhao, Z., Li, F., & Zhang, J. (2022). Effects of organic and inorganic fertilization on soil organic carbon and enzymatic activities. Agronomy, 12(12), 3125. https://doi.org/10.3390/agronomy12123125
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