Morphological and pathogenic characterization of Fusarium species causing common bean root rot in Uganda

Authors

  • Samuel Erima National Agricultural Research Organization - National Crops Resources Research Institute, Namulonge, P.O. Box 7084 Kampala, Uganda, College of Agricultural and Environmental Sciences, Makerere University, Kampala, P.O. Box 7062 Kampala, Uganda
  • Moses Nyine Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
  • Richard Edema College of Agricultural and Environmental Sciences, Makerere University, Kampala, P.O. Box 7062 Kampala, Uganda
  • Allan Nkuboye National Agricultural Research Organization - National Crops Resources Research Institute, Namulonge, P.O. Box 7084 Kampala, Uganda
  • Justine Nakibuule National Agricultural Research Organization - National Crops Resources Research Institute, Namulonge, P.O. Box 7084 Kampala, Uganda
  • Pamela Paparu National Agricultural Research Organization - National Crops Resources Research Institute, Namulonge, P.O. Box 7084 Kampala, Uganda

DOI:

https://doi.org/10.25081/jsa.2024.v8.8849

Keywords:

Common bean, Disease incidence, Disease prevalence, Fusarium root rot, Pathogenicity

Abstract

Fusarium root rot (FRR) of common bean occurs in Africa, Central and South America, and causes yield losses of up to 86%. Recently, FRR-like wilt symptoms were observed in Uganda’s agroecology zones. To identify the causal pathogen, we conducted surveys in seven agroecology zones to determine the prevalence and incidence of the reported disease. During the surveys, diseased roots were collected for pathogen isolation.  Fungal strains were characterized using colony color, mycelial growth rate and microscopic structures such as conidia and microconidia. The pathogenicity of 99 strains on five bean varieties was determined in artificially inoculated soils in the screenhouse. Based on field symptoms, the observed wilting was identified to be Fusarium root rot, the prevalence of which varied across agroecologies, with the highest (95%) in the Karamoja pastoral zone (KP) and the lowest (40%) in West Nile farming system (WN). Similarly, the incidence of FRR was highest (87%) in KP, and lowest (20%) in WN. Fusarium strains differed in growth rate, colony color, shape and size of microscopic structures. All evaluated strains were pathogenic on common bean and caused severities of 0.9 to 98.3%. Further studies are required to identify the isolated strains at the species level using molecular tools.

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Published

23-05-2024

How to Cite

Erima, S., Nyine, M., Edema, R., Nkuboye, A., Nakibuule, J., & Paparu, P. (2024). Morphological and pathogenic characterization of Fusarium species causing common bean root rot in Uganda. Journal of Scientific Agriculture, 8, 7–14. https://doi.org/10.25081/jsa.2024.v8.8849

Issue

Volume 8, 2024

Section

Articles

Copyright (c) 2024 Samuel Erima, Moses Nyine, Richard Edema, Allan Nkuboye, Justine Nakibuule, Pamela Paparu

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.