Comparison of Near-infrared Spectroscopy with other options for total carotenoids content phenotyping in fresh cassava roots

  • Wilfred Abincha Makerere University, P.O Box 7062, Kampala, Uganda & Mount Kenya University, P.O Box 4441, Kisii, Kenya
  • Robert Kawuki National Crops Resources Research Institute, P.O. Box 7084, Kampala, Uganda
  • Ugochukwu Ikeogu Cornell University, Ithaca, NY, USA
  • Justus Obara Egerton University, P. O Box 536 Egerton 20115, Kenya
  • Yasmin Hussin Makerere University, P.O Box 7062, Kampala, Uganda
  • Chiedozie Egesi Cornell University, Ithaca, NY, USA
  • Peter Kulakow International Institute of Tropical Agriculture, Ibadan, Nigeria
  • Ismail Rabbi International Institute of Tropical Agriculture, Ibadan, Nigeria
  • Betty-Elizabeth Owor Makerere University, P.O Box 7062, Kampala, Uganda
  • Elizabeth Parkes International Institute of Tropical Agriculture, Ibadan, Nigeria

Abstract

This study compared the relationship of different phenotyping methods including iCheckTM CAROTENE (iCheck), Chromameter, colour chart and visible/near-infrared spectroscopy (Vis/NIRS) used in quantifying total carotenoids content (TCC) in fresh cassava roots. Using a total of 194 cassava clones harvested from the International Institute of Tropical Agriculture (IITA), Ibadan, we compared the repeatability precision, accuracy of measurement and correlations of these phenotyping methods. From the results, Vis/NIRS-analyzed TCC had high and positive correlations with Chromameter and Color chart (r = 0.91 and 0.71, respectively). On the other hand, the result revealed somewhat moderate correlation (r = 0.67) between Vis/NIRS and iCheck measurements. Vis/NIRS, iCheck and chromameter methods gave high and nearly equal heritability estimates (0.95, 0.98 and 0.98, respectively) illustrating high repeatability precision of these methods; an indication that they can be used for germplasm selection in the early stages of breeding. Conversely, with Bland-Altman plot at 95% confidence level, the accuracy of iCheck was not comparable with that of Vis/ NIRS. The information derived from this analysis directly contributes towards the genetic improvement of root quality traits in cassava and facilitates the sharing of data across cassava breeding consortium.

Keywords: Phenotyping, Near-infrared spectroscopy, Carotenoids, iCheckTM CAROTENE, Cassava.

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