Establishment of Callus and Cell Suspension Cultures of Helicteres isora L.

  • Samrin Shaikh Department of Biotechnology, Modern College of Arts, Science and Commerce (Savitribai Phule Pune University), Ganeshkhind, Pune 411016, India
  • Varsha Shriram Department of Botany, Prof. Ramkrishna More College, (Savitribai Phule Pune University), Akurdi, Pune 411044, India
  • Tushar Khare and Vinay Kumar Department of Biotechnology, Modern College of Arts, Science and Commerce (Savitribai Phule Pune University), Ganeshkhind, Pune 411016, India

Abstract

Helicteres isora L. (Malvaceae) is a medicinal plant highly used in traditional therapeutic practices. It has shown wide-spectrum therapeutic activities including anti-plasmodial, hypoglycemic, hypolipidemic, hepatoprotective, antinociceptive, antioxidant and anti-HIV. Present investigation was undertaken with an objective of establishment of cell suspension cultures of this plant which can be further used for in vitro production of desired secondary metabolites and their further scale-up. Seed dormancy was broken using sulfuric acid and seedlings were raised in vitro. MS medium supplemented with 2,4-D (0.5 mg/L) produced maximum callus from the nodal explants. The callus produced was used as an explant for the establishment of suspension cultures. MS medium without any supplement was proved best for the establishment of cell suspension cultures of H. isora. To the best of our knowledge, this is the first report on H. isora cell suspension culture establishment.    

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How to Cite
Shaikh, S., V. Shriram, and T. K. and Vinay Kumar. “Establishment of Callus and Cell Suspension Cultures of Helicteres Isora L.”. Research in Plant Biology, Vol. 8,  , pp. 01-04, doi:https://doi.org/10.25081/ripb.2018.v8.3366.
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