In vitro propagation of Solidago virgaurea L. through nodal culture

Abstract

A reliable in vitro propagation protocol has been established from nodal segmentsof a highly valuable medicinal plant Solidago virgaurea L. The explants were cultured onMurashige and Skoog’s medium augmented with different concentrations andcombinations of plant growth regulators for shoot bud initiation and multiplications.Highest frequency of shoot proliferation (91.8 ± 0.64) and maximum number (12.1 ± 0.20) pernode was observed in Murashige and Skoog’s medium augmented with4.5 mg/l of Kinetinin combination with 0.5 mg/l of Indole -3- Acetic acid. Highest frequency (91.7 ± 1.43) ofrooting and maximum number (3.8 ± 0.28) of rootlet per shoot let was achieved onMurashige and Skoog’s medium fortified with 4.5 mg/l of Indole -3- Acetic acid incombination with 0.5 mg/l of Benzyl -6- amino purine. Regenerated plants weresuccessfully transferred to field (78%).