Isolation and characterization of extreme halophilic bacterium Salinicoccus sp. JAS4 producing extracellular hydrolytic enzymes

Authors

  • Jayachandra S Y Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.
  • Anil Kumar S Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.
  • Merley D P Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.
  • Sulochana M B Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.

Keywords:

Salinicoccus sp., Extracellular enzymes, 16S rRNA, Phylogenetic analysis

Abstract

A extreme halophilic bacterium, strain JAS4 was isolated from the Arabal soil of west coast of Karnataka, India. The isolate is Gram positive, strictly aerobic, ferments several carbohydrates and has motile, coccoid shaped cells and non sporing, catalase- and oxidase- positive, that grew in presence of 2-25% (w/v) NaCl and at pH 6.5-11, with optimum growth at 10%(w/v) NaCl, with an optimum growth temperature of 340C, has potential to produce the extracellular enzymes such as Amylase, Protease, Inulinase and Gelatinase, but production of lipase was found to be negative. The phenotypic studies and genotypic analysis by 16S rRNA analysis showed that the bacterium belonged to the genera Salinicoccus of 98% BLAST sequence similarity and it is named as Salinicoccus sp. JAS4 and phylogenetic study was carried out using Mega5 software.   

Downloads

Download data is not yet available.

Author Biographies

Jayachandra S Y, Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.

Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.

Anil Kumar S, Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.

Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.

Merley D P, Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.

Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.

Sulochana M B, Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.

Department of Studies and Research in Biotechnology, Gulbarga University, Gulbarga, Karnataka, India-585106.

Published

20-05-2012

How to Cite

Y, J. S., S, A. K., P, M. D., & B, S. M. (2012). Isolation and characterization of extreme halophilic bacterium Salinicoccus sp. JAS4 producing extracellular hydrolytic enzymes. Recent Research in Science and Technology, 4(4). Retrieved from https://updatepublishing.com/journal/index.php/rrst/article/view/888

Issue

Volume 4, Issue 4 (2012)

Section

Articles

Most read articles by the same author(s)