Artificial pollination and in vitro asymbiotic seed germination in garden orchid Spathoglottis plicata Blume (Orchidaceae)

Authors

  • Uma Thakur Department of Botany, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur 440033, India
  • Nitin Dongarwar Department of Botany, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur 440033, India

Keywords:

Spathoglottis plicata, artificial pollination, asymbiotic germination, green house acclimatization.

Abstract

Pollination biology of Garden orchid Spathoglottis plicata was investigated. Due absence of appropriate pollinator in our area the seed setting was absent or was very low. Artificial pollination (Hand pollination) in S. plicata was studied and seed setting was observed by self and cross pollination. Flowers of S. plicata were pollinated and subsequent capsule development was carefully monitored. Hand pollination of orchid flowers provides an opportunity to discuss floral morphology and associated reproductive biology. Capsules were harvested 60 days after the pollination prior to capsule dehiscence. In vitro asymbiotic germination was 87.3% when seeds were cultured on MS media. The effect of BA and NAA hormones were also observed on seed germination. Seedlings were healthy and stronger when cultured on MS media supplemented with hormones. The cultured seedlings were ready to greenhouse acclimatization after 110 days.

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Author Biographies

Uma Thakur, Department of Botany, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur 440033, India

Department of Botany, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur 440033, India

Nitin Dongarwar, Department of Botany, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur 440033, India

Department of Botany, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur 440033, India

Published

14-03-2012

How to Cite

Thakur, U., & Dongarwar, N. (2012). Artificial pollination and in vitro asymbiotic seed germination in garden orchid Spathoglottis plicata Blume (Orchidaceae). Recent Research in Science and Technology, 4(2). Retrieved from https://updatepublishing.com/journal/index.php/rrst/article/view/864

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Section

Articles