Biochemcial Changes in Powery Mildew Infected Leaves of Abelmoschus esculentus

Abstract

Four types of leaf samples A₁, A_{2 ,} B₁ and B₂ were taken from healthy and infected plants; and their free amino acids, sugars and organic acid contents were analyzed chromatographically. In case of free amino acids lysine, arginine, asparatic acid, glutamic acid, glycine and phenylatanine were found in all. There was no qualitative difference except for lysine, which gets totally consumed during the course of pathogenesis. Quantitatively was a general increase in amino acid content in B1 sample. In all three sugars were detected, they were fructose, sucrose and raffinose in all the four sample. There was a gradual decrease in the concentration of fructose and sucrose during the pathogenesis. While raffinose was maximum A₁ in sample. Organic acid detected in these samples were sebacic acid, adipic acid, succinic acid, maleic acid and citric acid. Succinic acid was totally missing in B₂ sample. While adipic and citric acid were maximum in B₂ sample. The maximum concentration of sebacic acid was A₁ sample and was least in B₂. The concentration of maleic acid was maximum in A₂ sample.