Effect of Carbon, Nitrogen, Sulphur, Phosphorus, Antibiotic and Vitamin Sources on Hydrolytic Enzyme Production by Storage Fungi

Abstract

In present investigation emphasis is given on to screen the lipolytic activity of storage fungi. Abnormal safflower seeds of PBNS-12 and Bhima varieties were collected from Marathwada region of Maharashtra state. Dominant fungi were isolated from abnormal oilseeds on Potato Dextrose Agar (PDA). Total twenty fungi were isolated. Out of that lipase enzyme activity of ten dominant fungi other than Aspergillus sp. was studied by using different nutritional sources like carbon, nitrogen, phosphorus, sulphur, antibiotic and vitamin sources. It was found that carbon sources like fructose and sucrose induces lipase activity while starch, lactose and carboxyl methyl cellulose (CMC) inhibits lipase activity. Nitrogen sources like casein and peptone which are organic forms stimulated maximum lipase enzyme production of storage fungi. Sulphur sources like calcium sulphate and ferrus sulphate reduced the lipase enzyme production by storage fungi while, phosphorus source like di-sodium hydrogen ortho-phosphate, ammonium phosphate and potassium di-hydrogen ortho-phosphate stimulated lipase enzyme production. Antibiotic like ampicillin, norfloxacin and tetracycline reduced the lipase production of storage fungi. Lipase activity of storage fungi was reduced in presence of vitamin source like riboflavin while, folic acid and vitamin C stimulated the lipase enzyme production.