Simultaneous Spectrophotometric Estimation of 11β,17α-dihydroxy -4-pregnene-3,20-dione- 21-succinate and its 1(2)-Dehydrogenated Product During Bioconversion by Comamonas acidovorans MTCC 3364

  • Kirti Pawar1*, Neelam Lad1 and S. P. Pawar2

Abstract

A spectrophotometric method for simultaneously estimating 11β,17α-dihydroxy-4-pregnene-3,20-dione 21-succinate and its 1(2)-dehydrogenated product has been developed using sulphuric acid chromogens. The method was used to follow the progress of bioconversion during C-1(2)-dehydrogenation by Comamonas acidovorans MTCC 3364.

Keywords: Steroid 1(2)-dehydrogenation; Simultaneous estimation; Steroid bioconversion; Hydrocortisone; Prednisolone estimation

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Author Biography

Kirti Pawar1*, Neelam Lad1 and S. P. Pawar2
1Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, New Vallabh Vidya Nagar, Anand- 388 121, Gujarat, India2A R College of Pharmacy, Vallabh Vidya Nagar, Anand- 388 121, Gujarat, India
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How to Cite
Neelam Lad1 and S. P. Pawar2K. P. “Simultaneous Spectrophotometric Estimation of 11β,17α-Dihydroxy -4-Pregnene-3,20-Dione- 21-Succinate and Its 1(2)-Dehydrogenated Product During Bioconversion by Comamonas Acidovorans MTCC 3364”. Recent Research in Science and Technology, Vol. 3, no. 1, Dec. 2010, https://updatepublishing.com/journal/index.php/rrst/article/view/561.
Section
Microbiology