Intracellular Expression and Purification of a Recombinant Enzyme Involved in Bioconversion of Arteannuin B to Artemisinin in Escherichia coli Expression System

Authors

  • Jyotheeswara Reddy Eedula1, Parcha Sreenivasa Rao2, M. Lakshmi Narasu1*

Abstract

Streptomyces pactum produces an enzyme that converts Arteannuin B to Artemisinin that is an important antimalarial. The gene encoding this novel enzyme from Streptomyces pactum MTCC 3664 has been cloned and expressed in Escherichia coli BL21 (DE3). The enzyme is expressed in the form of inclusion bodies at 370C or in a soluble form at 280C. The recombinant enzyme was purified by Immobilized Metal Ion Affinity Chromatography (IMAC). The purified enzyme produced by constitutive expression was found to be functionally active as it showed the conversion of arteannuin B to artemisinin as detected by TLC and HPLC.

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Author Biography

Jyotheeswara Reddy Eedula1, Parcha Sreenivasa Rao2, M. Lakshmi Narasu1*

1Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Andhra Pradesh, India- 500 0852Department of Biotechnology, National Institute of Technology, Warangal, A.P, India- 506 004

Published

01-01-2011

How to Cite

Sreenivasa Rao2, M. Lakshmi Narasu1*, J. R. E. . P. (2011). Intracellular Expression and Purification of a Recombinant Enzyme Involved in Bioconversion of Arteannuin B to Artemisinin in Escherichia coli Expression System. Recent Research in Science and Technology, 3(1). Retrieved from https://updatepublishing.com/journal/index.php/rrst/article/view/560

Issue

Volume 3, Issue 1, 2011

Section

Biotechnology