Indolicidin – Antibacterial activity against bacterial pathogens isolated from ocular infections

Authors

  • G Nambirajan Department of Microbiology, M. R. Government Arts College, Mannargudi, India.
  • B Annamalai Research Scholar, Bharathiar University, Coimbatore – 641 046, India.
  • C S Shobana Research Scholar, Bharathiar University, Coimbatore – 641 046, India.
  • K Panneer Selvam Department of Microbiology, M. R. Government Arts College, Mannargudi, India.

Keywords:

Indolicidin, antimicrobial activity, pET 21a plasmid, IPTG

Abstract

Indolicidin is a novel/ promising antimicrobial peptide (a 13 amino acid cationic antimicrobial residue present in the form of cytoplasmic granules of bovine neutrophils) and observed with a broad spectrum of antimicrobial activity against bacteria, fungi, protozoa & even viruses. In the present study, Escherichia coli was transformed with pET 21a+ plasmid carrying indolicidin gene and was expressed. The crude extracts of indolicidin samples induced with varying IPTG concentrations (5mM and 20 mM/ ml of the medium) in Min A medium were checked for antibacterial activities against clinically important ocular bacterial pathogens such as E. coli, Klebsiella sp,, Pseudomonas sp., Acenitobacter sp., Staphylococcus aureus, coagulase negative Staphylococcus aureus, Streptococcus viridans, S. pneumoniae and S. pyogens and its activity was evaluated. 

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Author Biographies

B Annamalai, Research Scholar, Bharathiar University, Coimbatore – 641 046, India.

Research Scholar, Bharathiar University, Coimbatore – 641 046, India. 

C S Shobana, Research Scholar, Bharathiar University, Coimbatore – 641 046, India.

Research Scholar, Bharathiar University, Coimbatore – 641 046, India. 

K Panneer Selvam, Department of Microbiology, M. R. Government Arts College, Mannargudi, India.

Department of Microbiology, M. R. Government Arts College, Mannargudi, India.

Published

21-02-2015

How to Cite

Nambirajan, G., Annamalai, B., Shobana, C. S., & Panneer Selvam, K. (2015). Indolicidin – Antibacterial activity against bacterial pathogens isolated from ocular infections. Recent Research in Science and Technology, 6(2). Retrieved from https://updatepublishing.com/journal/index.php/rrst/article/view/1147

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