Effect of metal ions on amylase production

Sangeeta Jha; Himadri Rai; Richa Chattopadhyay; Veer Pal Kaur; M Indupriya; V Shanti

Authors

Sangeeta Jha Department of Microbiology and Biotechnology St.Thomas College, Bhilai (C.G.), India.
Himadri Rai Department of Microbiology and Biotechnology St.Thomas College, Bhilai (C.G.), India.
Richa Chattopadhyay Department of Microbiology and Biotechnology St.Thomas College, Bhilai (C.G.), India.
Veer Pal Kaur Department of Microbiology and Biotechnology St.Thomas College, Bhilai (C.G.), India.
M Indupriya Department of Microbiology and Biotechnology St.Thomas College, Bhilai (C.G.), India.
V Shanti Department of Microbiology and Biotechnology St.Thomas College, Bhilai (C.G.), India.

Keywords:

Apergillus and Amylase

Abstract

Amylase producing fungi were isolated from different soil samples. About 11 fungal isolates were isolated which were screened for Amylase production on starch agar medium using iodine test. Of the 11 isolates, three isolates showed clear zones and the isolate showing maximum clearance zone was selected for further studies. Identification of isolate showed that it belonged to Genus Aspergillus. The effect of different metal ions on the production and activity of Amylase was studied. Five different ions were used for the study. Starch agar medium was prepared with different metal ions and inoculated with the culture and incubated. It was seen that maximum mycelial growth was observed with MgSO4 followed by KNO3 but very less or negligible growth was seen when grown with CaCO3. Therefore, Mg ions were considered to be the best ion for optimum growth of the fungus and thus considered the best inducer for Amylase production as maximum enzyme activity was observed with Mg ions but Ca and Cu ions showed less activity and therefore might be showing inhibitory effect on Amylase production.