Analysis of interaction between Arbuscular Mycorrhizal fungi and their Helper bacteria by MILPA model

Abstract

Many recent researches carried out the research in mycorrhizal symbiosis to improve crop growth in the agriculture but they differ in their approaches like crops and the agricultural perspectives. The study focused on the interaction of Arbuscular mycorrhizal (AM) fungi and helper bacteria, influence maize growth through milpa as a model system to analyze soil enzyme activity of acid, alkaline phosphatase and signaling molecule of phospholipids fatty acid (PLFA) profile. Bioinoculants plays an important role in plant growth like nutrient mobilization, biocontrol and prevent the plants from stress. The maize crops were treated with bio inoculants such as Azospirillum, Azotobacter, Rhizobium,Pseudomonas and AM fungi. Significant diverse effects were observed with bioinoculants in the crops compared to control crop. The number spores formed was proportional to the rate of colonization.AM fungi association in plant roots helps the plants in nutrient uptake especially phosphorus (P) from soil materials. The enzyme activities were also found to influence the growth of the plant and phospholipids fatty acid (PLFA) profile influences the interaction between bacterium and AMF. Plant and phospholipids fatty acid analysis is asensitive and accurate method in determining microbial community structures, because it depends on living cell contents of microorganisms under in situ conditions. It confirmed with gas chromatographic analysis of PLFA to determination of the structure and total biomass of microbial community in treated soil samples.