An efficient protocol devised for rapid callus induction from leaf explants of Stephania wightii (Arn.) Dunn. - an endemic medicinal plant

Authors

  • U. Danya PG and Research Department of Botany, Kongunadu arts and science college (Autonomous), Coimbatore, Tamil Nadu
  • M.R. Udhayasankar PG and Research Department of Botany, Kongunadu arts and science college (Autonomous), Coimbatore, Tamil Nadu
  • K. Arumugasamy PG and Research Department of Botany, Kongunadu arts and science college (Autonomous), Coimbatore, Tamil Nadu
  • D. Punitha Department of Botany, Providence College for Women, Coonor, The Nilgiris, Tamil Nadu

Keywords:

Stephania wightii, callus, leaf explant and growth regulators

Abstract

Callus culture of Stephania wightii (Arn.) Dunn. was established from leaf explants.Different growth regulators greatly influenced the growth of callus cultures. Leaf derivedcallus grown on Murashige and Skooge (MS) medium fortified with different concentrations(0.1-2.5 mg/L) of Thiodiazuran (TDZ), 6-Benzyladenine (BA), 2, 4- DichlorophenoxyaceticAcid (2, 4-D) and α-Naphthalene Acetic Acid (NAA). Maximum callus growth was achievedin medium supplemented with TDZ + NAA (1.5+0.02 mg/L) was found to be more suitablethan other growth regulators used.

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Published

14-10-2012

How to Cite

Danya, U., Udhayasankar, M., Arumugasamy, K., & Punitha, D. (2012). An efficient protocol devised for rapid callus induction from leaf explants of Stephania wightii (Arn.) Dunn. - an endemic medicinal plant. Research in Plant Biology, 2(5). Retrieved from https://updatepublishing.com/journal/index.php/ripb/article/view/2542

Issue

Vol. 2 No. 5 (2012)

Section

Short Communications