In vitro propagation of Solidago virgaurea L. through nodal culture

Authors

  • John Peter Paul J Department of Plant Biology and Plant Biotechnology, St. Xavier’s College (Autonomous), Palayamkottai – 627 002, Tamil Nadu, India
  • I Revathy Department of Plant Biology and Plant Biotechnology, St. Xavier’s College (Autonomous), Palayamkottai – 627 002, Tamil Nadu, India
  • M Johnson Department of Plant Biology and Plant Biotechnology, St. Xavier’s College (Autonomous), Palayamkottai – 627 002, Tamil Nadu, India

Keywords:

in vitro, Clonal, Nodal segments, Medicinal plants

Abstract

A reliable in vitro propagation protocol has been established from nodal segmentsof a highly valuable medicinal plant Solidago virgaurea L. The explants were cultured onMurashige and Skoog’s medium augmented with different concentrations andcombinations of plant growth regulators for shoot bud initiation and multiplications.Highest frequency of shoot proliferation (91.8 ± 0.64) and maximum number (12.1 ± 0.20) pernode was observed in Murashige and Skoog’s medium augmented with4.5 mg/l of Kinetinin combination with 0.5 mg/l of Indole -3- Acetic acid. Highest frequency (91.7 ± 1.43) ofrooting and maximum number (3.8 ± 0.28) of rootlet per shoot let was achieved onMurashige and Skoog’s medium fortified with 4.5 mg/l of Indole -3- Acetic acid incombination with 0.5 mg/l of Benzyl -6- amino purine. Regenerated plants weresuccessfully transferred to field (78%).

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Published

14-08-2012

How to Cite

Paul J, J. P., I. Revathy, and M. Johnson. “In Vitro Propagation of Solidago Virgaurea L. Through Nodal Culture”. Research in Plant Biology, vol. 2, no. 4, Aug. 2012, https://updatepublishing.com/journal/index.php/ripb/article/view/2532.

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