Isolation and identification of flavonoid from in vitro and natural grown Nyctanthes arbor-tristis L. (Harsingar) - a medicinally important plant

Abstract

A rapid propagation protocol was established and optimized in vitro for Nyctanthesarbor-tristis L. a small ornamental tree with medicinal and aromatic properties. Nodalexplants were cultured on Murashige and Skoog (MS) medium supplemented with variousplant growth regulators: BAP and Kn in combination. Maximum frequency of shoot initiation(FSI) (1-2 wks) and mean shoot number (MSN) 3.02±0.437 were obtained on BAP + Kn (1.0 +0.1), whereas high mean shoot length (MSL) 2.9±0.356 was observed on BAP + Kn (2.0 + 0.5)Elongated microshoots (3-4 wks) were successfully rooted and regenerated plants weresimultaneously hardened and acclimatized. An approximately 91% survival rate wasrecorded. Natural leaf, stem, in vitro leaf and stem were subsequently extracted in 80%methanol and were subjected to thin layer chromatography along with the standard.