Sequencing and analysis of invariant surface glycoprotein (ISG) gene from Trypanosoma evansi dog isolate

Authors

  • G R Rudramurthy National Institute of Veterinary Epidemiology and Disease Informatics
  • Pinaki P Sengupta National Institute of Veterinary Epidemiology and Disease Informatics
  • J N Lakkundi Parasitology Department, Veterinary College, KVAFSU, Bidar, Karnataka, India
  • M Ligi National Institute of Veterinary Epidemiology and Disease Informatics
  • P E D’Souza Parasitology Department, Veterinary College, KVAFSU, Hebbal, Bangalore 560024, Karnataka, India.
  • H Rahman National Institute of Veterinary Epidemiology and Disease Informatics

Abstract

Trypanosoma (T) evansi causes a chronic wasting disease called “surra†in cattle, buffaloes, horses, canines, felines etc. In the present study T.evansi isolated from dog from Karnataka state in India was used to sequence invariant surface glycoprotein (ISG) gene and the obtained sequence was analyzed to elucidate its relationship with other isolates/ species. The  ISG gene sequences obtained from four recombinant clones revealed the open reading frame (ORF) of  1521  nucleotide (nt) encoding a polypeptide of 506 amino acids (aa) and belongs ISG-75 gene family. Sequence analysis revealed 92-99% and 65-99% similarity at nt and aa levels, respectively with other isolates / species and belongs to the RoTat 1.2 strain

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Published

28-12-2014

How to Cite

Rudramurthy, G. R., Sengupta, P. P., Lakkundi, J. N., Ligi, M., D’Souza, P. E., & Rahman, H. (2014). Sequencing and analysis of invariant surface glycoprotein (ISG) gene from Trypanosoma evansi dog isolate. Research in Biotechnology, 6(1). Retrieved from https://updatepublishing.com/journal/index.php/rib/article/view/2654

Issue

Vol. 6 No. 1 (2015)

Section

Research Articles