Genetic Structure and Molecular Phylogeny Analysis of Bracon brevicornis Wesmael, a Larval Parasitoid of Coconut Black Headed Caterpillar, Opisina arenosella Walker

Authors

  • K. Rukhsana University of Calicut
  • C. D. Sebastian University of Calicut

Keywords:

Opisina arenosella, parasitoid, Bracon brevicornis, phylogeny, cytochrome oxidase I gene

Abstract

Bracon brevicornis (Hymenoptera: Braconidae) a larval parasitoid of O. arenosella and the present study reveals molecular barcoding and phylogeny analysis of B. brevicornis. The adult B. brevicornis gave excellent control of larvae of O. arenosella. We have PCR amplified and sequenced partial fragment of cytochrome oxidase subunit I gene (COI) of B. brevicornis (Genbank Accession: KM 216266) isolated from Kerala and its  phylogenetic status was studied. In Partial COI DNA sequence of B. brevicornis has is 0.16% difference to that of  Habrobracon hebetor (KJ 627789) and 0.48% difference to other Braconinae species (FN 662379, FN 662380, FN 662381, FN 662382). Leucine is the most abundant amino acid in partial COI sequence which is 20.3 % of total amino acids.


Downloads

Download data is not yet available.

Author Biographies

K. Rukhsana, University of Calicut

Research Scholar

C. D. Sebastian, University of Calicut

Associate Professor, Department of Zoology

Published

18-05-2015

How to Cite

Rukhsana, K., & Sebastian, C. D. (2015). Genetic Structure and Molecular Phylogeny Analysis of Bracon brevicornis Wesmael, a Larval Parasitoid of Coconut Black Headed Caterpillar, Opisina arenosella Walker. Research in Biotechnology, 6(3). Retrieved from https://updatepublishing.com/journal/index.php/rib/article/view/2472

Issue

Vol. 6 No. 3 (2015)

Section

Research Articles