Clove (Syzygium aromaticum): Processing, bioactives, and applications

Processing and biological applications of clove

Authors

  • M Madhumitha National Institute of Food Technology, Entrepreneurship and Management, Thanjavur (NIFTEM-T), Thanjavur – 613 005, Tamil Nadu
  • K Abhijith National Institute of Food Technology, Entrepreneurship and Management, Thanjavur (NIFTEM-T), Thanjavur – 613 005, Tamil Nadu
  • Baskaran Nagarethinam National Institute of Food Technology, Entrepreneurship and Management, Thanjavur (NIFTEM-T), Thanjavur – 613 005, Tamil Nadu.
  • Vignesh Sivanandham National Institute of Food Technology, Entrepreneurship and Management, Thanjavur (NIFTEM-T), Thanjavur – 613 005, Tamil Nadu.

DOI:

https://doi.org/10.25081/josac.2025.v34.i2.9937

Keywords:

Bioactive compounds, clove, eugenol, functional foods, food processing

Abstract

Clove (Syzygium aromaticum (L.). Merr & L.M. Perry) is a widely used spice valued for its rich aroma and potent biological properties. This article highlights the traditional and modern processing techniques of clove in a detailed manner, including harvesting, destalking, drying, and storage, with a focus on quality retention. It explores both volatile (e.g., eugenol) and non-volatile (e.g., tannins, sterols) compounds responsible for its therapeutic efficacy. The paper also presents an overview of clove’s pharmacological applications, such as antimicrobial, antidiabetic, anticancer, antioxidant, and anti-inflammatory activities. Technological advancements in extraction and nanoencapsulation have enhanced its application in functional foods, pharmaceuticals, and green packaging. This review emphasizes the significance of optimized processing and bioactive preservation for maximizing the medicinal and commercial potential of clove.

Downloads

Download data is not yet available.

References

Abdelmuhsin A A, Sulieman A M E, Salih Z A, Al-Azmi M, Alanaizi N A, Goniem A E & Alam M J 2025 Clove (Syzygium aromaticum) Pods: Revealing Their Antioxidant Potential via GC-MS Analysis and Computational Insights. Pharmaceuticals 18(4): 504. https://doi.org/10.3390/ph18040504

Abdul Kareem F B, Elumalai A, Anandharaj A, Sivanandham V & Nagarethinam B 2024 Exploring the preservation efficiency of cured betel leaf essential oil in augmenting the quality of fruit juice: a comprehensive evaluation of physicochemical, microbial, and sensory parameters. J. Food Sci. Technol. 61(10): 1862-1873. https://doi.org/10.1007/s13197-024-05963-9

Adio A A, Ademola S G, Akinwumi A O, Arasi K K, Shittu M D, Ogunesa S T, Oyekan O G & Ogunbiyi O D 2024 performance and antioxidant status of stored eggs from hens fed dietary ginger and clove. Niger. J. Anim. Prod. : 802-805. https://doi.org/10.51791/njap.vi.5726

Ahmad M F 2020 Ganoderma lucidum: A rational pharmacological approach to surmount cancer. J. Ethnopharmacol. 260: 113047. https://doi.org/10.1016/j.jep.2020.113047

Ahmad Wani S, Singh A & Kumar P 2022 Spice Bioactive Compounds. CRC Press, Boca Raton. https://doi.org/10.1201/9781003215387

Alanazi A K, Alqasmi M H, Alrouji M, Kuriri F A, Almuhanna Y, Joseph B & Asad M 2022 Antibacterial Activity of Syzygium aromaticum (Clove) Bud Oil and Its Interaction with Imipenem in Controlling Wound Infections in Rats Caused by Methicillin-Resistant Staphylococcus aureus. Molecules 27(23). https://doi.org/10.3390/molecules27238551

Anand Kumar Singh 2009 Anti-stress activity of hydro-alcoholic extract of Eugenia caryophyllus buds (clove). Indian J. Pharmacol. 1(41): 28-31. https://doi.org/https://doi.org/10.4103/0253-7613.48889

Astani A, Reichling J & Schnitzler P 2011 Screening for antiviral activities of isolated compounds from essential oils. Evidence-Based Complementary and Alternative Medicine 2011: 253643. https://doi.org/10.1093/ecam/nep187

Atsumi T, Iwakura I, Fujisawa S & Ueha T 2001 Reactive oxygen species generation and photo-cytotoxicity of eugenol in solutions of various pH. Biomaterials 22(12): 1459- 1466. https://doi.org/10.1016/S0142-9612(00)00267-2

Bachir R G & Benali M 2012 Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pac. J. Trop. Biomed. 2(9): 739-742. https://doi.org/10.1016/S2221-1691(12)60220-2

Bakkali F, Averbeck S, Averbeck D & Idaomar M 2008 Biological effects of essential oils - A review. J. Food Chem. Toxicol. 46(2): 446- 475. https://doi.org/10.1016/j.fct.2007.09.106

Barajas-Álvarez P, Castillo-Herrera G A, Guatemala-Morales G M, Corona-González R I, Arriola-Guevara E & Espinosa-Andrews H 2021 Supercritical CO2-ethanol extraction of oil from green coffee beans: optimization conditions and bioactive compound identification. J. Food Sci. Technol. 58(12): 4514- 4523. https://doi.org/10.1007/s13197-020-04933-1

Basava Prasad A R, Arunkumar A, Vignesh S, Chidanand D V & Baskaran N 2022 Exploring the nutritional profiling and health benefits of Palmyra palm haustorium. S. Afr. J. Bot. 151: 228- 237. https://doi.org/10.1016/j.sajb.2022.01.027

Basava Prasad A R, Vignesh S, Elumalai A, Anandharaj A, Chidanand D V & Baskaran N 2023 Nutritional and pharmacological properties of palmyra palm. Food Humanit. 1: 817-825. https://doi.org/10.1016/j.foohum.2023.07.030

Baskaran N, Manoharan S, Balakrishnan S & Pugalendhi P 2010 Chemopreventive potential of ferulic acid in 7,12-dimethylbenz[a]anthracene-induced mammary carcinogenesis in Sprague–Dawley rats. Eur. J. Pharmacol. 637 (1-3): 22-29. https://doi.org/10.1016/j.ejphar.2010.03.054

Baskaran N, Manoharan S, Karthikeyan S & Prabhakar M M 2012 Chemopreventive potential of coumarin in 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. Asian Pac. J. Cancer Prev. 13 (10): 5273-5279. https://doi.org/10.7314/APJCP.2012.13.10.5273

Baskaran N, Selvam G S, Yuvaraj S & Abhishek A 2018 Parthenolide attenuates 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis. Mol. Cell. Biochem. 440 (1-2): 11-22. https://doi.org/10.1007/s11010-017-3151-5

Benencia F & Courrges M C 2000 In vitro and in vivo activity of eugenol on human herpesvirus. Phytother. Res. 14 (7): 495-500. https://doi.org/10.1002/1099-1573(200011)14:7<495::AID-PTR650>3.0.CO;2-8

Benkirane C, Allay A, Ben Moumen A, Belhaj K, Melhaoui R, Mansouri F, Abid M, Serghini-Caid H & Elamrani A 2022 Aqueous enzymatic extraction of safflower (Carthamus tinctorius L.) seed oil: a preliminary study. E3S Web Conf. 337: 04010. https://doi.org/10.1051/e3sconf/202233704010

Cansian R L, Vanin A B, Orlando T, Piazza S P, Puton B M S, Cardoso R I, Gonçalves I L, Honaiser T C, Paroul N & Oliveira D 2017 Toxicity of clove essential oil and its ester eugenyl acetate against Artemia salina. Braz. J. Biol. 77 (1): 155-161. https://doi.org/10.1590/1519-6984.12215

Chaieb K, Hajlaoui H, Zmantar T, Kahla-Nakbi A B, Rouabhia M, Mahdouani K & Bakhrouf A 2007 The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzygium aromaticum L., Myrtaceae): a short review. Phytother. Res. 21 (6): 501-506. https://doi.org/10.1002/ptr.2124

Charu S V, Chidanand D V, Ramesh M & Baskaran N 2024 Impact of cold plasma on pearl and barnyard millets’ microbial quality, antioxidant status and nutritional composition. Food Humanit. 2: 100238. https://doi.org/10.1016/j.foohum.2024.100238

Choudhary C, Vignesh S, Chidanand D V & Baskaran N 2025 Effect of different processing methods on nutrient, phytochemical composition and microbial quality of pearl millet. Food Humanit. 4: 100513. https://doi.org/10.1016/j.foohum.2025.100513

Cortés-Rojas D F, de Souza C R F & Oliveira W P 2014 Clove (Syzygium aromaticum): a precious spice. Asian Pac. J. Trop. Biomed. 4 (2): 90-96. https://doi.org/10.1016/S2221-1691(14)60215-X

Danthu P, Simanjuntak R, Fawbush F, Leong Pock Tsy J M, Razafimamonjison G, Abdillahi M M, Jahiel M & Penot E 2020 The clove tree and its products (clove bud, clove oil, eugenol): prosperous today but what of tomorrow’s restrictions? Fruits 75 (5): 224-242. https://doi.org/10.17660/th2020/75.5.5

Devi K P, Nisha S A, Sakthivel R & Pandian S K 2010 Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. J. Ethnopharmacol. 130 (1): 107-115. https://doi.org/10.1016/j.jep.2010.04.025

Dua A, Garg G, Nagar S, & Mahajan R 2014 Methanol extract of clove (Syzygium aromaticum Linn.) damages cells and inhibits growth of enteropathogens. J. Innov. Biol. 1(4): 200- 205.

El-Saber Batiha G, Alkazmi L M, Wasef L G, Beshbishy A M, Nadwa E H & Rashwan E K 2020 Syzygium aromaticum L. (Myrtaceae): Traditional uses, bioactive chemical constituents, pharmacological and toxicological activities. Biomolecules 10 (2): 202. https://doi.org/10.3390/biom10020202

Fadilah F, Yanuar A, Arsianti A & Andrajati R 2017 Phenylpropanoids, eugenol scaffold and its derivatives as anticancer agents. Asian J. Pharm. Clin. Res. 10 (3): 41. https://doi.org/10.22159/ajpcr.2017.v10i3.16071

Farooqi A A & Sreeramu B S 2005 Cultivation of Spice Crops. Universities Press, Hyderabad, India. https://books.google.com/books?id=7KPUlXxOYZAC&pgis=1

Fu Y, Zu Y, Chen L, Shi X, Wang Z, Sun S & Efferth T 2007 Antimicrobial activity of clove and rosemary essential oils alone and in combination. Phytother. Res. 21 (10): 989- 994. https://doi.org/10.1002/ptr.2179

Gertsch J, Leonti M, Raduner S, Racz I, Chen J Z, Xie X Q, Altmann K H, Karsak M & Zimmer A 2008 Beta-caryophyllene is a dietary cannabinoid. Proc. Natl. Acad. Sci. USA 105 (26): 9099- 9104. https://doi.org/10.1073/pnas.0803601105

Ghai R, Nagarajan K, Singh J, Swarup S & Keshari M 2016 Evaluation of antioxidant status in vitro and in vivo in hydro-alcoholic extract of Eugenia caryophyllus. Int. J. Pharmacol. Toxicol. 4 (1): 19-24. https://doi.org/10.14419/ijpt.v4i1.5880

Gülçin I 2004 Comparison of antioxidant activity of clove (Eugenia caryophyllata Thunb.) buds and lavender (Lavandula stoechas L.). Food Chem. 87 (3): 393- 400. https://doi.org/10.1016/j.foodchem.2003.12.008

Gunaseelan D, Ali M S, Albert A, Prabhakaran R, Beno D L & Nagarethinam B 2022 Biochemical and molecular anticancer approaches for Boerhaavia diffusa root extracts in oral cancer. J. Cancer Res. Ther. 18 (Suppl 2): S244- S252. https://doi.org/10.4103/jcrt.JCRT_932_20

Gupta C & Prakash D 2021 Comparative study of the antimicrobial activity of clove oil and clove extract on oral pathogens. Dent. Open J. 7 (1): 12-15. https://doi.org/10.17140/DOJ-7-144

Haidl G 2011 New WHO reference limits - revolution or storm in a teapot? Asian J. Androl. 13: 208-211. https://doi.org/10.1038/aja.2010.156

Han X & Parker T L 2017 Anti-inflammatory activity of clove (Eugenia caryophyllata) essential oil in human dermal fibroblasts. Pharm. Biol. 55 (1): 1619-1622. https://doi.org/10.1080/13880209.2017.1314513

Harinee S, Muthukumar K, Dahms H U, Koperuncholan M, Vignesh S, Banu R J, Ashok M & James R A 2019 Biocompatible nanoparticles with enhanced photocatalytic and anti-microfouling potential. Int. Biodeterior. Biodegrad. 145: 104790. https://doi.org/10.1016/j.ibiod.2019.104790

Hassan N 2023 Clove production - A case study in Kanyakumari, Tamil Nadu. ResearchGate Preprint. https://doi.org/10.13140/RG.2.2.33795.89129

Idowu O O 2021 Evaluation of selected organic residues as supplements on growth and yield of oyster mushroom, Pleurotus florida. Pan Afr. J. Life Sci. 5 (1). https://doi.org/10.36108/pajols/1202/50.0180

Idowu S, Adekoya A E, Igiehon O O & Idowu A T 2021 Clove (Syzygium aromaticum) spices: A review on their bioactivities, current use and potential application in dairy products. J. Food Meas. Charact. 15 (4): 3419- 3435. https://doi.org/10.1007/s11694-021-00915-9

Ijarotimi O S, Yinusa M A, Adegbembo P A & Adeniyi M D 2018 Chemical compositions, functional properties, antioxidative activities and glycaemic indices of raw and fermented tigernut tubers (Cyperus esculentus Lativum) flour. J. Food Biochem. 42 (5): e12591. https://doi.org/10.1111/jfbc.12591

Joseph B, Bhavadharani M, Lavanya M, Nivetha S, Baskaran N & Vignesh S 2024 Comparative analysis of LAB and non-LAB fermented millet drinks fortified with Chlorella sp. Food Bioeng. 3 (3): 352- 364. https://doi.org/10.1002/fbe2.12102

Joseph B, Bhavadharani M, Nivetha S, Lavanya M, J M R, Baskaran N & Vignesh S 2025 Synbiotic fermented barnyard millet drink: Exploring its nutritional profile, sensory attributes and bioactive health potentials. Food Chem. Adv. 6: 100872. https://doi.org/10.1016/j.focha.2024.100872

Kaur K, Kaushal S & Rani R 2019 Chemical composition, antioxidant and antifungal potential of clove (Syzygium aromaticum) essential oil, its major compound and its derivatives. J. Essent. Oil Bear. Plants 22 (5): 1195- 1217. https://doi.org/10.1080/0972060X.2019.1688689

Kembauw E, Mahulette A S, Kakisina A P, Tuhumury M T F, Umanailo M C B & Kembauw M G I 2021 Clove processing as a source of increasing business income in Ambon City. IOP Conf. Ser.: Earth Environ. Sci. 883 (1): 012091. https://doi.org/10.1088/1755-1315/883/1/012091

Kerala Agricultural University 2019 KAU Agri-infotech portal. Available at: www.celkau.in

Kim S S, Oh O J, Min H Y, Park E J, Kim Y, Park H J, Han Y N & Lee S K 2003 Eugenol suppresses cyclooxygenase-2 expression in lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells. Life Sci. 73 (3): 337- 348. https://doi.org/10.1016/S0024-3205(03)00288-1

Kiriti Aaradhana S, Arun Kumar E, Vignesh S, Chidanand D V & Baskaran N 2023 Evaluating the effects of different processing methods on the nutritional quality of bovine milk. Food Humanit. 1: 128-136. https://doi.org/10.1016/j.foohum.2023.05.005

Krishnan M, Dahms H U, Seeni P, Gopalan S, Sivanandham V, Jin-Hyoung K & James R A 2017 Multi metal assessment on biofilm formation in offshore environment. Mater. Sci. Eng. C 73: 743-755. https://doi.org/10.1016/j.msec.2016.12.062

Krishnan M, Subramanian H, Dahms H U, Sivanandham V, Seeni P, Gopalan S, Mahalingam A & Rathinam A J 2018 Biogenic corrosion inhibitor on mild steel protection in concentrated HCl medium. Sci. Rep. 8 (1): 2609. https://doi.org/10.1038/s41598-018-20718-1

Kuiate J R, Bessière J M, Zollo P H A & Kuate S P 2006 Chemical composition and antidermatophytic properties of volatile fractions of hexanic extract from leaves of Cupressus lusitanica Mill. from Cameroon. J. Ethnopharmacol. 103 (2): 160- 165. https://doi.org/10.1016/j.jep.2005.07.022

Kumar P, Lokesh V, Doddaraju P, Kumari A, Singh P, Meti B S, Sharma J, Gupta K J & Manjunatha G 2021 Greenhouse and field experiments revealed that clove oil can effectively reduce bacterial blight and increase yield in pomegranate. Food Energy Secur. 10 (4). https://doi.org/10.1002/fes3.305

Kunicka-Styczyńska A, Tyfa A, Laskowski D, Plucińska A, Rajkowska K & Kowal K 2020 Clove oil (Syzygium aromaticum L.) activity against Alicyclobacillus acidoterrestris biofilm on technical surfaces. Molecules 25 (15): 3334. https://doi.org/10.3390/molecules25153334

Lakshmipraba J, Arunachalam S, Gandi D A, Thirunalasundari T, Vignesh S & James R A 2017 Interaction of polyethyleneimine-anchored copper(II) complexes with tRNA studied by spectroscopy methods and biological activities. Luminescence 32 (3): 309- 316. https://doi.org/10.1002/bio.3179

Lakshmipraba J, Arunachalam S, Riyasdeen A, Dhivya R, Vignesh S, Akbarsha M A & James R A 2013 DNA/RNA binding and anticancer/antimicrobial activities of polymer-copper(II) complexes. Spectrochim. Acta A Mol. Biomol. Spectrosc. 109: 23- 31. https://doi.org/10.1016/j.saa.2013.02.020

Lavanya M, Nivetha S, Baskaran N & Vignesh S 2024 Exploration of eco-benign antifoulant in combating seafood-associated biofilms: An in vitro study on impacts of myrobalan mediated FeNPs against biofilming SS-316 metal coupon. Int. J. Food Eng. 20 (8): 601-616. https://doi.org/10.1515/ijfe-2023-0302

Leela, N. K.; Sapna, V. P. Clove. In Chemistry of Spices; CABI: 2008; pp 146- 164. https://doi.org/10.1079/9781845934057.0146

Manoharan S, Baskaran N & Rajasekaran D 2011 Coumarin protects 7,12-dimethylbenz(a)anthracene-induced genotoxicity in the bone marrow cells of golden Syrian hamsters. Int. J. Nutr. Pharmacol. Neurol. Dis. 1 (2): 167. https://doi.org/10.4103/2231-0738.84209

Manoharan S, Vasanthaselvan M, Silvan S, Baskaran N, Kumar Singh A & Vinoth Kumar V 2010 Carnosic acid: A potent chemopreventive agent against oral carcinogenesis. Chem. Biol. Interact. 188 (3): 616- 622. https://doi.org/10.1016/j.cbi.2010.08.009

Mustafa A A 2025 Antimicrobial and antioxidant activities and GC/MS analysis of clove oil (Syzygium aromaticum). Sci. Med. Life J. 11 (1). https://doi.org/10.52981/smlj.v11i1

Musthafa K S, Hmoteh J, Thamjarungwong B & Voravuthikunchai S P 2016 Antifungal potential of eugenyl acetate against clinical isolates of Candida species. Microb. Pathog. 99: 19-29. https://doi.org/10.1016/j.micpath.2016.07.012

Nuñez L & D’Aquino M 2012 Microbicide activity of clove essential oil (Eugenia caryophyllata). Braz. J. Microbiol. 43 (4): 1255-1260. https://doi.org/10.1590/S1517-83822012000400003

Ogata M, Hoshi M, Urano S & Endo T 2000 Antioxidant activity of eugenol and related monomeric and dimeric compounds. Chem. Pharm. Bull. 48 (10): 1467- 1469. https://doi.org/10.1248/cpb.48.1467

Olatunji T L, Kazeem A A, Olatunji H C O & F C 2013 Aphrodisiac effect of ethanol extract of Piliostigma thonningii leaf on male albino Wistar rats. J. Appl. Pharm. Sci. 3 (10): 130-135. https://doi.org/10.7324/JAPS.2013.31023

Otunola G A 2022 Culinary spices in food and medicine: An overview of Syzygium aromaticum (L.) Merr. & L. M. Perry (Myrtaceae). Front. Pharmacol. 12. https://doi.org/10.3389/fphar.2021.793200

Pandey V K, Srivastava S, Ashish, Dash K K, Singh R, Dar A H, Singh T, Farooqui A, Shaikh A M & Kovacs B 2024 Bioactive properties of clove (Syzygium aromaticum) essential oil nanoemulsion: A comprehensive review. Heliyon 10 (1): e22437. https://doi.org/10.1016/j.heliyon.2023.e22437

Pandiyarajan T, Udayabhaskar R, Vignesh S, James R A & Karthikeyan B 2013 Synthesis and concentration dependent antibacterial activities of CuO nanoflakes. Mater. Sci. Eng. C 33 (4): 2020- 2024. https://doi.org/10.1016/j.msec.2013.01.021

Pei C, Zhang Y, Wang P, Zhang B, Fang L, Liu B & Meng S 2019 Berberine alleviates oxidized low-density lipoprotein-induced macrophage activation by downregulating galectin-3 via the NF-κB and AMPK signaling pathways. Phytother. Res. 33 (2): 294–308. https://doi.org/10.1002/ptr.6217

Periasamy J, Krishnamoorthy S, Nagarethinam B & Sivanandham V 2023 Food wastes as a potential hotspot of antibiotic resistance: Synergistic expression of multidrug resistance and ESBL genes confer antibiotic resistance to microbial communities. Environ. Monit. Assess. 195 (6): 783. https://doi.org/10.1007/s10661-023-11335-1

Perumal A B, Nambiar R B, Sellamuthu P S & Emmanuel R S 2021 Use of modified atmosphere packaging combined with essential oils for prolonging post-harvest shelf life of mango (cv. Banganapalli and cv. Totapuri). LWT 148: 111662. https://doi.org/10.1016/j.lwt.2021.111662

Phothisuwan S, Preechatiwong W & Matan N 2020 Enhancement of antibacterial activity of essential oil vapor released from a paper egg tray in combination with UV-C radiation against pathogenic bacteria on chicken eggs. J. Food Process. Preserv. 44 (10). https://doi.org/10.1111/jfpp.14794

Prakash C, Tyagi J, Singh K V, Kumar G & Sharma D 2025 Eugenol attenuates aluminium-induced neurotoxicity in rats by inhibiting the activation of STAT3 and NF-κB. Metab. Brain Dis. 40 (1): 87. https://doi.org/10.1007/s11011-024-01526-1

Prashar A, Locke I C & Evans C S 2006 Cytotoxicity of clove (Syzygium aromaticum) oil and its major components to human skin cells. Cell Prolif. 39 (4): 241- 248. https://doi.org/10.1111/j.1365-2184.2006.00384.x

Pruthi J S 1998 Spices and Condiments. National Book Trust, New Delhi, India.

Juma S R, Joseph C C & Nyandoro S S 2024 Chemical constituents of the essential oils from Uvariodendron mbagoi. Bull. Chem. Soc. Ethiop. 38 (4): 1027-1035. https://doi.org/10.4314/bcse.v38i4.17

Radünz M, da Trindade M L M, Camargo T M, Radünz A L, Borges C D, Gandra E A & Helbig E 2019 Antimicrobial and antioxidant activity of unencapsulated and encapsulated clove (Syzygium aromaticum L.) essential oil. Food Chem. 276: 180-186. https://doi.org/10.1016/j.foodchem.2018.09.173

Raja Rajeshwari E, Sathanya P S, Vignesh S, Chandrasekar V & Baskaran N 2025 Valorization of Borassus flabellifer sprout peel: Synthesis and characterization of carboxymethyl cellulose for biodegradable packaging. Biomass Convers. Biorefin. https://doi.org/10.1007/s13399-025-06500-0

Rajeshwari E R, Sathanya P S, Vignesh S & Baskaran N 2024 Palmyra and coconut haustorium: A comprehensive review on nutritional composition, bioactive potential, value-added products and its health benefits. Food Chem. Adv. 5: 100836. https://doi.org/10.1016/j.focha.2024.100836

Raji Y, Fadare O O, Adisa R A & Salami S A 2006 Comprehensive assessment of the effect of Sphenocentrum jollyanum root extract on male reproductive activity in albino rats. Reprod. Med. Biol. 5 (4): 283-292. https://doi.org/10.1111/j.1447-0578.2006.00154.x

Ratri P J, Ayurini M, Khumaini K & Rohbiya A 2020 Clove oil extraction by steam distillation and utilization of clove buds waste as potential candidate for eco-friendly packaging. J. Bahan Alam Terbarukan 9 (1): 47- 54. https://doi.org/10.15294/jbat.v9i1.24935

Rodrigues E & Carlini E A 2004 Plants used by a Quilombola group in Brazil with potential central nervous system effects. Phytother. Res. 18 (9): 748- 753. https://doi.org/10.1002/ptr.1535

Ruyati S 2024 Evaluation of the anti-diabetic properties of clove essential oil. Biol. Sci. 4 (2). https://doi.org/10.55006/biolsciences.2024.4205

Shanmugam M, Baskaran N, Manoharan S & Muneeswaran M 2010 Chemopreventive efficacy of berberine in 7,12-dimethylbenz[a]anthracene (DMBA) induced skin carcinogenesis in Swiss albino mice. Int. J. Res. Pharm. Sci. 1 (4): 521- 529. www.pharmascope.org.

Shukla V, Mendiratta S K, Zende R J, Agrawal R K & Jaiswal R K 2020 Effects of chitosan coating enriched with Syzygium aromaticum essential oil on quality and shelf-life of chicken patties. J. Food Process. Preserv. 44 (11). https://doi.org/10.1111/jfpp.14870

Sung B, Prasad S, Yadav V R & Aggarwal B B 2012 Cancer cell signaling pathways targeted by spice-derived nutraceuticals. Nutr. Cancer 64 (2): 173-197. https://doi.org/10.1080/01635581.2012.630551

Suresh D, Manjunatha H & Srinivasan K 2007 Effect of heat processing of spices on the concentrations of their bioactive principles: Turmeric (Curcuma longa), red pepper (Capsicum annuum) and black pepper (Piper nigrum). J. Food Compos. Anal. 20 (3- 4): 346- 351. https://doi.org/10.1016/j.jfca.2006.10.002

Tajuddin, Ahmad S, Latif A & Qasmi I A 2004 Effect of 50% ethanolic extract of Syzygium aromaticum (L.) Merr. & Perry (clove) on sexual behaviour of normal male rats. BMC Complement. Altern. Med. 4 (1): 17. https://doi.org/10.1186/1472-6882-4-17

Thapa D, Richardson A J, Zweifel B, Wallace R J & Gratz S W 2019 Genoprotective effects of essential oil compounds against oxidative and methylated DNA damage in human colon cancer cells. J. Food Sci. 84 (7): 1979- 1985. https://doi.org/10.1111/1750-3841.14665

Thomas L, Anees K & Muhammed Nissar V A 2023 The economic landscape of clove production in India: Challenges and opportunities. J. Spices Aromat. Crops 32 (2): 161- 169. https://doi.org/10.25081/josac.2023.v32.i2.8880

Tian C, Zhang T, Wang L, Shan Q & Jiang L 2014 The hepatoprotective effect and chemical constituents of total iridoids and xanthones extracted from Swertia mussotii Franch. J. Ethnopharmacol. 154 (1): 259-266. https://doi.org/10.1016/j.jep.2014.04.018

Vignesh G, Arunachalam S, Vignesh S & James RA 2012 BSA binding and antimicrobial studies of branched polyethyleneimine- copper(II) bipyridine/phenanthroline complexes. Spectrochim. Acta A Mol. Biomol. Spectrosc. 96: 108-116. https://doi.org/10.1016/j.saa.2012.05.009

Vignesh G, Pradeep I, Arunachalam S, Vignesh S, Arthur James R, Arun R & Premkumar K 2016 Biological and protein-binding studies of newly synthesized polymer- cobalt(III) complexes. Luminescence 31: 533- 543. https://doi.org/10.1002/bio.2992

Vignesh G, Sugumar K, Arunachalam S, Vignesh S & Arthur James R 2013 A comparative study on the binding of single and double chain surfactant- cobalt(III) complexes with bovine serum albumin. Spectrochim. Acta A Mol. Biomol. Spectrosc. 113: 415- 422. https://doi.org/10.1016/j.saa.2013.04.123

Vignesh S, Muthukumar K & Arthur James R 2012 Antibiotic resistant pathogens versus human impacts: A study from three eco-regions of the Chennai coast, southern India. Mar. Pollut. Bull. 64: 790-800. https://doi.org/10.1016/j.marpolbul.2012.01.015

Yang L, Jiang Y, Zhang Z, Hou J, Tian S & Liu Y 2020 The anti-diabetic activity of licorice, a widely used Chinese herb. J. Ethnopharmacol. 263: 113216. https://doi.org/10.1016/j.jep.2020.113216

Yashwant CP, Rajendran V, Krishnamoorthy S, Nagarathinam B, Rawson A, Anandharaj A & Sivanandham V 2023 Antibiotic resistance profiling and valorization of food waste streams to starter culture biomass and exopolysaccharides through fed-batch fermentations. Food Sci. Biotechnol. 32: 863- 874. https://doi.org/10.1007/s10068-022-01222-9

Published

08-04-2026

How to Cite

Madhumitha, M., Abhijith, K., Nagarethinam, B., & Sivanandham, V. (2026). Clove (Syzygium aromaticum): Processing, bioactives, and applications: Processing and biological applications of clove. Journal of Spices and Aromatic Crops, 34(2), 133–150. https://doi.org/10.25081/josac.2025.v34.i2.9937