Biocontrol activity of Streptomyces isolate SDSRO-13 against Colletotrichum spp. causing anthracnose disease of chilli (Capsicum annuum L.)
Screening of Streptomyces sp. against anthracnose of chilli
DOI:
https://doi.org/10.25081/josac.2023.v32.i2.8879Keywords:
Actinomycetes, 16S rRNA, Colletotrichum sp., chilli, anthracnose disease, biocontrol activityAbstract
A total of 18 Streptomyces species were isolated from the rhizospheric soil of maize. Among 18 isolates, SDSRO-13 showed strong inhibition against Colletotrichum spp. of chilli. SDSRO-13 was identified as Streptomyces sp. based on morphological, biochemical and 16S rRNA partial gene sequence analysis. In this study, two strains of Colletotrichum sp. were used for pathogenicity test. For in vivo antifungal activity, spore suspension of the test pathogens were used on the fresh chilli fruit by making a small puncture on the fruit, in completely sterile conditions and incubated for up to 21 days. The chilli fruits treated with SDSRO-13 and test pathogens showed no disease symptoms. The infected fruits were subjected for Koch’s postulate test and they showed the similar morphological features compared to the pure cultures of the pathogens. We conclude that Actinomycetes, especially members of the genus Streptomyces are potential biocontrol agents which offer safer alternative management strategy to control the chilli fungal pathogens.
Downloads
References
Altschul S F, Gish W, Miller W, Myers E W, Lipman D J 1990 Basic local alignment search tool. J. Mol. Biol. 215(3): 403-410.
Augustine S K, Bahvsar S P & Kapadnis B P 2005 A non polyene antifungal antibiotic from Streptomyces albidoflavus PU23. J. Biosci. 30(2): 201–211.
Darby A C, Chandler S M, Welburn, S C & Douglas A E 2005 Aphid-symbiotic bacteria cultured in insect cell lines. Applied Environmental Microbiology. 71(8): 4833-4839.
Haritha R, Siva Kumar K, Jagan Mohan Y S & Ramana T 2010 Amylolytic and Proteolytic actinobacteria isolated from Marine Sediments of Bay of Bengal. Int. J. Microbiol. Res. 1(2): 37–44.
Hassan O, Jeon J Y & Chang T 2018. Molecular and morphological characterization of Colletotrichum species in the Colletotrichum gloeosporioides complex associated with persimmon anthracnose in South Korea. Plant Diseases. 102(5): 1015–1024.
Hyi J K, Eun J L, Sung H P, Hoi S L & Namhyun C. 2014 Biological Control of Anthracnose (Colletotrichum gloeosporioides) in Pepper and Cherry Tomato by Streptomyces sp. A1022. J. Agric Sci. 6(2): 1–10.
Jeffrey L S H, Umi K M S, Nor A A R, Khozirah S & Halizah H 2015 Streptomyces ambofaciens S2 - A Potential Biological Control Agent for Colletotrichum gleosporioides the Causal Agent for Anthracnose in Red Chilli Fruits. Int. J. Plant Pathol. Microbiol. 4: 1–7.
Jisha M.S, Linu M.S & Sreekumar J 2018 Induction of systemic resistance in chilli (Capsicum annuum L.) by Pseudomonas aeruginosa against anthracnose pathogen Colletotrichum capsici. J. Trop. Agric. 56 (2): 153–166.
Karlin S & Altschul S F 1990 Methods for assessing the statistical significance of molecular sequence features by using general scoring schemes. Proceedings of the National Academy of Sciences. USA. 87: 2264–2268.
Khucharoenphaisan K, Sripairoj N, & Sinma K 2013 Isolation and identification of actinomycetes from termite’s gut against human pathogen. Asian Journal of Animal and Veterinary Advances. 7: 68–73.
Kunova A, Bonaldi M, Saracchi M, Pizzatti C & Chen X 2016 Selection of Streptomyces against soil borne fungal pathogens by a standardized dual culture assay and evaluation of their effects on seed germination and plant growth. BMC Microbiology. 16: 272. 1–11.
Linu M S, Jisha M S & Jisha M S 2017 In vitro control of Colletotrichum capsici induced chilli anthracnose by fungicides and biocontrol agent. International Journal of Applied and Pure Science and Agriculture. 3(5): 1–7.
Mesta S C & Onkarappa R 2017 Characterization and antimicrobial potential of marine actinomycetes from estuaries of Uttara Kannada district, Karnataka. Journal of Drug Delivery & Therapeutics 7(6): 27–37.
Mills P R, Sreenivasaprasad S & Brown A E 1992 Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR. FEMS Microbiology Letter. 98: 137–144.
Mohammadipanah F & Wink J 2016 Actinobacteria from Arid and Desert Habitats: Diversity and Biological Activity. Frontiers in Microbiology. 6:1541. 1–10.
Myers E W & Miller W 1988 Optimal alignments in linear space. Computational Applied Biosciences. 4(1): 1–11.
Pakdeevaraporn P, Wasee S, Taylor P J & Mongkolporn O 2005 Inheritance of resistance to anthracnose caused by Colletotrichum capsici in capsicum. Plant Breeding. 124(2): 206–208.
Pavithra S, Akila R, Rajinimala N, Gangai Selvi R & Kannan R 2019 Plant extraction mediated mitigation of chilli fruit rot caused by Colletotrichum spp. Journal of Pharmacognosy and Phytochemistry 8(4): 2879–2883.
Petriccione M, Zampella L, Mastrobuoni F & Scortichini M 2017 Occurrence of copper-resistant Pseudomonas syringae pv. syringae strains isolated from rain and kiwifruit orchards also infected by P. s. pv. actinidiae. European Journal of Plant Pathology. 149: 953–968.
Rahman A U & Humainy A A 2011 Taificidin 1 and Taificidin 2, two antimicrobial agents isolated from the fermentation broth of Streptomyces roseodistaticus TA15 and Streptomyces lavendofoliae TA17. Research journal of Microbiology. 6(4): 328–342.
Reddy M K, Srivastava A, Kumar S R, Kumar R, Chawda N A, Ebert A W & Vishwakarma M 2014 Chilli (Capsicum annuum L.) breeding in india. SABRAO Journal of Breeding and Genetics. 46 (2): 160–173.
Rhavena G L, Maria I S F & Marcos A S 2019 Streptomyces griseocarneus R132 controls phytopathogens and promotes growth of pepper (Capsicum annuum). 138: 1–9
Rodríguez M, Torres M, Blanco L, Béjar V, Sampedro I & Llamas I 2020 Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6. Scientific Reports. 10: 1–12.
Sabaratnam S & Traquair J A 2015 Mechanism of antagonism by Streptomyces griseocarneus (strain Di944) against fungal pathogens of greenhouse-grown tomato transplants. Canadian J. Plant Path. 37:197–211.
Sahin N & Ugur A 2003 Investigation of the Antimicrobial activity of some Streptomyces isolates. Turkish Journal of Biology. 27: 79–84.
Sandra Pacios M, Cristobal N, Aguilar G, Olga B, Alvarez-Perez, Raul R H, Mónica C G, Roberto A V, Juan A, Ascacio V, Mayela G S & Anna I 2021 Application of Streptomyces Antimicrobial Compounds for the Control of Phytopathogens. Frontiers in Sustainable Food Systems. 5: 1–13.
Saputri E K, Amalia D & Wahyuni W S 2021 Streptomyces sp. has different efficacy to control two different pathogens. J. earth environ. sci.759: 1–6.
Sharma M & Manhas R K 2020 Purification and characterization of salvianolic acid B from Streptomyces sp. M4 possessing antifungal activity against fungal phytopathogens. Microbiological research. 237: 1–14
Shrivastava P, Kumar. R & Yandigeri M S 2017 In vitro biocontrol activity of halotolerant Streptomyces aureofaciens K20: A potent antagonist against Macrophomina phaseolina (Tassi) Goid. Saudi J. Bio Sci. 24: 192–199.
Sun D, Liu C, Zhu J & Liu W 2017 Connecting metabolic path-ways: sigma factors in Streptomyces spp. Frontiers in Microbiology. 8:2546. 1–7.
Tatsuya O, Minori M, Tetsuya M, Masahiro O, Shinhiro K & Takashi A 2018 In vitro and In vivo Assay for Assessment of Biological Control of Streptomyces sp KT. J. Plant Stud. 7(1): 1–14.
Vashistha A & Chaudhary A 2019 Effects of biocontrol agents and heavy metals in controlling soilborne phytopathogens. Int. J. Herb. Med. 7: 41–45.
Veerendra G, Arvinder K, Pramod K F & Himanshu S G 2017 Comparative studies on isolation of Colletotrichum capsici causing anthracnose disease of chilli. Int. J. Chem. Stud. 5(6): 744–747.
Waksman S A & Henrici A T 1943 The nomenclature and classification of the actinomycetes. J. Bacteriol 46: 337–341.
Zee K Y & Vu T A 2018 Effect of Trichoderma sp. on Anthracnose Disease of Stored Chilli. Borneo J. Resour. Sci. Technol. 8(2): 90–10.
Zhuang X, Congting G, Chenghui P, Zhiyan W, Junwei Z, Yue S & Chongxi L 2020 Characterization of a Novel Endophytic Actinomycete, Streptomyces physcomitrii sp. nov. and Its Biocontrol. Potential Against Ralstonia solanacearum on Tomato. Microorganisms. 8: 1–12.
Published
How to Cite
Issue
Section
Copyright (c) 2023 D S Savita, R Onkarappa
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
.