Kanamycin sensitivity of cultured tissues of Piper nigrum L.
Abstract
The kanamycin sensitivity for callus growth was studied in vitro in a cultivar of black pepper (Piper nigrum) using cotyledons as explants to investigate the suitability of kanamycin resistance as a selectable marker for Agrobacterium mediated transformation. Callus formation was completely inhibited at 50 ug ml-1 and above concentrations of kanamycin suggesting that 50 ug ml-1 is the minimum concentration needed to select the transformed tissues.
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Published
22-07-2017
How to Cite
VELUTHAMBI, B. S. K. (2017). Kanamycin sensitivity of cultured tissues of Piper nigrum L. Journal of Spices and Aromatic Crops, 3(2), 158–160. Retrieved from https://updatepublishing.com/journal/index.php/josac/article/view/4448
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Section
Research Notes