In vitro culture of Piper attenuatum Ham. (Piperaceae): Callus induction and plant regeneration

Authors

  • K MADHUSUDHANAN, B A RAHIMAN, B SANDHYA

Abstract

Callus induction and plant regeneration of Piper attenuatum was achieved from stem explants in Murashige and Skoog and Gamborg's basal media supplemented with 2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, Kinetin, benzyladenine and palmyarh (Borassus flabelliferl endosperm extract. Root induction was obtained from stem explants in Murashige and Skoog medium supplemented with a-naphthaleneacetic acid, b-naphthoxyacetic acid, indole-3-acetic acid, benzyladenine, kinetin and coconut water. Shoot development was achieved in in vitro rooted stem segments planted in Murashige and Skoog's medium supplemented with indole-3-acetic acid, benzyladenine and also in indole-3-acetic acid and kinetin. The plantlets were successfully established in soil. This is the first report of in vitro culture of P. attenuatum.

 

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Published

23-12-1996

How to Cite

SANDHYA, K. M. B. A. R. B. (1996). In vitro culture of Piper attenuatum Ham. (Piperaceae): Callus induction and plant regeneration. Journal of Spices and Aromatic Crops, 5(2), 127–130. Retrieved from https://updatepublishing.com/journal/index.php/josac/article/view/4385

Issue

Section

Research Notes