Direct regeneration of plant lets from immature inflorescenee of ginger (Zingiber officinale Rosc.) by tissue culture

Abstract

Immature inflorescences of ginger when cultured on MS revised medium supplemented with 10 mg 1-1 BA and 0.2 mgl-1 2, 4-D re~ulted in the conversion of floral buds into vegetative buds and these later developed into plantlets dil',ectly without intervening callus phase. About 38% of the buds produced multiple shoots ranging from 5-25. When individual flowers were cultured separately on the same medium plantlets developed from the ovary in 45% of cases. These plantlets were made to develop good root system on MS medium with 1 mgl-1 NAA. These rooted plantlets established easily in the soil. In some of the cuI tures the ovaries developed into fruits.