Evaluation of electrophoretic patterns of soluble proteins in salt stressed potato callus
Abstract
Assessment of genetic diversity based on various markers plays a key role in crop breeding programs. In this study, protein patterns of callus, produced from three potato cultivar (Sante, Savalan, and Agria) and grown under salinity stress were investigated. Potato seedlings were generated in vitro and then explants were collected and transferred onto MS media containing 5 mg/l 2, 4-D, and 2 mg/l kinetin to callus induction. Callus was placed onto new MS media containing 0, 25, 50, 75, and 100 mM NaCl. A 1-month-old callus was evaluated in terms of protein patterns using vertical electrophoresis. The results indicated that potato cultivars are divided into two main groups. Sante cultivar grown under no salt stress was placed in one group, and Agria cultivar grown under different concentrations of NaCl put in a separate group. According to the results, most of protein bands were appeared in 6.5-29 kDa regions. In general, Savalan cultivar produced more protein bands than Sante or Agria.
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