Field Bio-Efficacy of Entomopathogenic Nematode-Based Integrated Management of White Grubs in Arecanut

Authors

  • Rajkumar ICAR – Central Plantation Crops Research Institute, Kasaragod-671124, Kerala, India
  • Jaganathan D. ICAR- Central Tuber Crops Research Institute, Thiruvananthapuram-695017, Kerala, India
  • Shivaji Hausrao Thube ICAR- Central Cotton Research Institute Nagpur-441108, Maharashtra, India
  • Surekha ICAR – Central Plantation Crops Research Institute, Kasaragod-671124, Kerala, India
  • Prathibha V.H. ICAR – Central Plantation Crops Research Institute, Kasaragod-671124, Kerala, India
  • Nagaraja N. R. ICAR- Central Plantation Crops Research Institute, Regional Station, Vittal- 574243, Dakshina Kannada, Karnataka, India
  • Vinayaka Hegde ICAR – Central Plantation Crops Research Institute, Kasaragod-671124, Kerala, India

Abstract

The white grubs (root grubs), a soil-dwelling coleopteron insect pest are major problem for arecanut cultivation in Western Ghats of India. A three year field evaluation (2015– 2017) was conducted to assess the efficacy of integrated white grub management practices, which included the soil drench of a liquid suspension of local isolate of the entomopathogenic nematode(EPN), Steinernema carpocapsae, in combinations with other IPM practices. The experiment aimed to develop an integrated pest management system against white grubs in arecanut and to evaluate its impact on arecanut yield and related attributes. The integrated approach, comprising soil drainage, collection and destruction of grubs by digging palm basins (up to 10cm depth and 60cm from the trunk), soil application of neem cake at 2kg/palm, insecticide application (imidacloprid at 0.25ml/L of water), along with soil drenching of liquid suspension of EPN, S. carpocapsae at 20lakh IJs/palm,  resulted in a significant reduction in grub population (86  to 92%) and an increase in dry arecanut yield (164-332 kgs/acre) by 2017. In contrast, prior to treatment (2015), these gardens had the highest root grub population (11.6grubs/palm) and the lowest yields (48.6–238kgs/acre). These results demonstrate the effectiveness of EPN-based integrated pest management practices for white grub management in arecanut.

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References

Andalo, V., Santos, V., Moreira, G. F., Moreira, C. C. and Moino Junior, A. 2010.

Evaluation of entomopathogenic nematodes under laboratory and greenhouses conditions

for the control of Spodoptera frugiperda. Ciencia Rural. 40(9):1860–1866.

https://doi.org/10.1590/S0103-84782010005000151.

Bhatnagar, A. 2011. Susceptibility of eggs, pupae and adults of white grub, Maladera

insanabilis (Brenske) to entomopathogenic nematode, Heterorhabditis bacteriophora

Poinar. Indian Journal of Entomology, 73(4):360-364.

Cantelo, W.W. and Nickle, W.R. 1992. Susceptibility of prepupae of the Colorado potato

beetle (Coleoptera:Chrysomelidae) to entomopathogenic nematodes (Rhabditida:

Steinernematidae, Heterorhabditidae).Journal of Entomology Science, 27: 37-43

Chandrika Mohan, Josephrajkumar, A., Shivaji, H. Thube, Saneera, E. K. and Rajkumar

Pests and their management in arecanut. In: Mani M (eds). Trends in horticultural

entomology. Springer Nature Singapore Pte Ltd, Book (1553 pages).

https://doi.org/10.1007/978-981-19-0343-4 . pp 1529-1544.

Dutky, S. R, Thompson, L. V. and Cantwe, G. E. 1964. A technique for the mass

propagation of the DD-136 nematode. Journal of Insect Pathology, 6:417-422.

Gangadhara Naik, B., Maheshwarappa, H. P., Rajkumar, M., Kalleshwaraswamy, C. M.,

Gowdra Nagamma and Latha S. 2019. Evaluation of entomopathogenic nematodes for

the management of white grub, Leucopholis lepidophora Blanchard (Coleoptera:

Scarabaeidae). Journal of Entomology and Zoology Studies, 7(1):09-13.

Grewal, P. S. 2002. Formulation and application technology. In: Gaugler, R. (Ed.).

Entomopathogenic nematology, CABI, New York, NY, pp.265-288.

Hanumanthaswamy, B. C., Nagarajappa Adivappar and Pradeep Gopakkali 2017. Impact of

frontline demonstration on arecanut root grub management. International Journal of

Current Microbiology and Applied Sciences, 6(8):3422-3425.doi:

https://doi.org/10.20546/ijcmas.2017.608.410 .

Hominick, W. M. and Reid, A. P. 1990. Perspectives on entomopathogenic nematology. In:

Gaugler, R. & Kaya, H. K. (eds) Entomopathogenic nematodes in biological control.

CRC Press, Boca Raton, pp 327–345.

Jagadeesh Patil, Rajkumar and Kesavan Subhaharan 2015. Synergism of entomopathogenic

nematode and imidacloprid: A curative tool to coconut white grub, Leucopholis

coniophora (Coleoptera: Melolonthinae). Vegetos, International Journal of Plant

Research, 28(1):184-190.

Jeer Vinayaka, Patil, R. R. and Prabhu, S. T. 2018. Field evaluation of EC formulations of

Metarhizium anisopliae (Meschinikoff) Sorokin and few insecticides against arecanut

white grub, Leucopholis lepidophora, Blanchard. Journal of Entomology and Zoology

Studies, 6(2):1034-1037.

Kalleshwaraswamy, C. M., Adarsha, S. K., Naveena, N. L. and Sharanabasappa 2015.

Incidence of arecanut white grubs (Leucopholis spp.) in hilly and coastal regions of

Karnataka, India. Current Biotica, 8: 423-424.

Karunakar, G., Easwaramoorthy, S. and David, H. 2000. Host Parasitic interactions between

two species of white grubs infesting sugarcane and two species of EPNs. Sugar

Technology, 2:12-16.

Kaya, H. K. and Stock, S. P. 1997. Techniques in insect nematology, In: Lacey L.A. (ed).

Manual of techniques in insect pathology. Academics press, New York, pp.281-324.

Kaya, H. K. and Gaugler, R. 1993. Entomopathogenic nematodes. Annual Review of

Entomology, 38:181-206.

Khan, Y. S. and Javed, N. 2018. Entomopathogenic nematode survey, persistence in soil,

reproductive potential and their effects on Meloidogyne incognita. Egyptian Journal of

Agronematology, 17(2):109-120.

Khatri-Chhetri, H. B., Timsina, G. P., Manandhar, H. and Moens, M. 2011. Potential of

Nepalese entomopathogenic nematodes as biocontrol agents against Holotrichia

longipennis Blanch. (Coleoptera: Scarabaeidae). Journal of Pest Science, 84:457–469.

https://doi.org/10.1007/s10340-011-0370-5 .

Klein, M. G. 1990. Efficacy against soil inhibiting insect pests, In:Gaugler, R. Kaya, H.

K.(Eds.). Entomopathogenic nematodes in biological control. CRC Press, Boca Raton,

Florida, pp. 195-214.

Koppenhofer, A. M. and Fuzy, E. M. 2003. Biological and chemical control of the asiatic

garden beetle, Maladera castanea (Coleoptera; Scarabaeidae). Journal of Economic

Entomology, 96: 1076-1082.

Krishnayya, P. V. and Grewal, P. S. 2002. Effect of neem and selected fungicides on viability

and virulence of the entomopathogenic nematode Steinernema feltiae. Biocontrol Science

Technology, 12:259-266.

Nair, C. P. R. and Daniel, M. 1982. Pests of arecanut In: K.V.A. Bavappa, M. K. Nair and T.

Premkumar (Eds). The arecanut palm (Areca catechu Linn.) CPCRI, Kasargod (India).

pp: 162-184.

Padmanabhan, B., Mariamma Daniel and Srimannarayana, G. 1997. Evaluation of plant

products oil cake against white grub Leucopholis burmeisteri. Journal of Economic

Entomology, 59:362-365.

Padmanaban, B. and Daniel, M. 2003. Biology and bionomics of palm white grub

Leucopholis burmeisteri. Indian Journal of Entomology, 65: 444-452.

Patil, J., Rajkumar, M. and Subhaharan, K. 2014. Virulence of Steinernema carpocapsae and

Heterorhabditis indica against coconut rhinoceros beetle, Oryctes rhinoceros L.

(Scarabaeidae: Coleoptera). Indian Journal of Nematology, 44:73–81.

Patil, J., Linga, V., Vijayakumar, R., Subaharan, K., Navik, O., Bakthavatsalam, N.,

Mhatre, P. H. and Sekhar, J. 2022. Biocontrol potential of entomopathogenic nematodes

for the sustainable management of Spodoptera frugiperda (Lepidoptera: Noctuidae) in

maize. Pest Management Science, 54 (7):257–278.

Rajamani, S. and Nambiar, K. K. N. 1970. Pests of arecanut palm. Indian Farming, 20:23

Rajkumar, Rachana K. E., Rajesh M. K., Sabana, A. A., Nagraja, N. R., Shahin, S. and

Subaharan, K. 2016. Molecular identification of entomopathogenic nematode isolate and

its virulence to white grub, Leucopholis burmeisteri (Coleoptera: Scarabaeidae). Vegetos,

:4. doi:10.4172/2229-4473.1000187.

Rajkumar, Jagadeesh Patil, Pervez Rashid and Subhaharan Kesavan 2019. Management of

white grub (Leucopholis burmesteri) infesting arecanut through entomopathogenic

nematodes under field conditions. Annals of Plant Protection Sciences( An international

Journal of Crop Protection), 27(1):112-116.

Roopa, S. Patil 2017. Eco-friendly management of arecanut root grub ( Leucopholis

lepidophora Blanchard) in hilly tracts of Uttar Kannada, Karnataka. Journal of Krishi

Vigyan, 5(2) : 32-34. DOI : 10.5958/2349-4433.2017.00007.1.

Shabbir, K., Shivanna, B. K., Bhopale, S. P. and Jayaroa, B. 2014. Survey for root grub

incidence and their natural enemies. In: arecanut gardens in Shimoga district. Trends in

Biosciences, 7(18):2657-2661.

Shahina, F. and Salma, J. 2011. Pakistani strains of entomopathogenic nematodes as a

biological control against stored grain pest, T. castaneum. Pakistan Journal of

Nematology, 29(1): 25-34.

Shankarnarayanan, C., Somasekhar, N. and Singaravelu, B. 2006. Bio-control ppotential of

entomopathogenic nematodes Heterorhabditis and Steinernema against pupae and adults

of white grub, Holotrichia serrata F. Sugar Technology, 8(4):268-27.

Shang Ping Kung, Randy Gaugler and Harry, K. Kaya 1990. Soil type and entomopathogenic

nematode persistence. Journal of Invertebrate Pathology, 55(3):401-406.

Shapiro-Ilan, D. J., Gouge, D. H. and Koppenhofer, A. M. 2002. Factors affecting

commercial success: case studies in cotton, turf and citrus, In; Gaugler, R. (Ed.),

Entomopathogenic nematology. CABI Publishing, Wallingford, UK, pp.333-356.

Shapiro-Ilan, D. I., Gouge, D. H., Piggott, S. J. and Fife, J. P. 2006 Application technology

and environmental considerations for use of entomopathogenic nematodes in biological

control. Biological Control, 38(1):124–133. https:// doi. org/ 10.1016/j. bioco ntrol. 2005.

005

Stewart, J. G., Boiteau, G. and Kimpinski, J. 1998. Management of late-season adults of the

Colorado potato beetle (Coleoptera: Chrysomelidae) with entomopathogenic nematodes.

Canadian Entomology, 130: 509-514

Strong, D. R. 2002. Population of entomopathogenic nematodes in food webs. In: Gaugler R.

(ed). Entomopathogenic nematology. CABI Publishing, Oxon, UK. pp. 225-240.

Subaharan, K., Vidyasagar, P. S. P. V. and Mohammed Basheer, B. M. 2001. Bio-efficacy of

insecticide against white grub, Leucopholis lepidophora Blanch infesting arecanut palm.

Indian Journal of Plant Protection, 29(1&2):25-29.

Tarasco E., Kary N. E., Fanelli E., Mohammadi D., Xingyue L., Mehrvar A., De Luca F. and

Troccoli A. 2020. Modified bait insect technique in entomopathogens survey from the

arasbaran biosphere reserve (Iran). Redia 103:129–132

Thurston, G. S., Kaya, H. K., Burlando, T. M. and Harrison, R. E. 1993. Milky disease

bacterium as a stressor to increase susceptibility of scarabaeid larvae to an ento-

mopathogenic nematode. Journal of Invertebrate Pathology, 61: 167 172.

Thurston, G. S., Kaya, H. K. and Gaugler, R. 1994. Characterizing the enhanced

susceptibility of milky disease infected scarabaeid grubs to entomopathogenic nema

todes. Biological Control, 4: 67-73.

Vélez-Arango, A. M., Arango, R. E., Villanueva, D., Aguilera, E. and Saldamando, C. I.

Identification of Spodoptera frugiperda biotypes (Lepidoptera: Noctuidae) through

using mitochondrial and nuclear markers. Revista Colombiana de Entomologia, 34:145–

Veeresh, G. K., Vijayendra, M., Reddy, N. V. M. and Rajanna, C. 1982. Bio-ecology and

management of arecanut white grubs (Leucopholis sp.) (Coleoptera; Scarabaeidae:

Melolonthinae). Journal of Soil Biology and Ecology, 2:78–86.

White, G. F. 1927. A method for obtaining infective nematode larvae from cultures. Science,

: 302-303.

Published

17-12-2025

How to Cite

Rajkumar, D., J., Thube, S. H., Surekha, V.H., P., N. R., N., & Hegde, V. (2025). Field Bio-Efficacy of Entomopathogenic Nematode-Based Integrated Management of White Grubs in Arecanut. Journal of Plantation Crops, 53(1). Retrieved from https://updatepublishing.com/journal/index.php/JPC/article/view/9851

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Research Articles