Identification and utilization of informative EST-SSR markers for genetic purity testing of coconut hybrids
Coconut palms are categorized into two forms, viz., â€˜tallsâ€™ and â€˜dwarfsâ€™ which are being utilized to produce hybrids through theÂ process of inter-varietal or intra-varietal crosses. Hybrid coconut seedlings are generally identified and selected based onÂ morphological traits by plant breeders, which is quite difficult and requires expertise. Even minor errors in identification mayÂ adversely affect breeding programs in coconut, which is spread over many decades. In this study, we have utilized thirty EST-SSRÂ markers, derived from existing coconut leaf transcriptome data, for screening polymorphism between eighteen coconut parentalÂ lines. The polymorphic primers capable of differentiating the parental palms were then utilized successfully for assessment ofÂ purity of hybrids derived from these parents. Thus, the current study demonstrates the utility of EST-SSR markers in determining
the genetic purity of hybrids in coconut.
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