Microsatellite-inferred genetic architecture of oil palm germplasm

Authors

  • H. P. Bhagya ICAR-Directorate of Cashew Research, Puttur, Dakshina Kannada-574202, Karnataka, India
  • B. Kalyana Babu ICAR-Indian Institute of Oil Palm Research, Pedavegi-534450, Andhra Pradesh, India

DOI:

https://doi.org/10.25081/jpc.2026.v54.i1.10079

Abstract

The oil palm is a plant that produces vegetable oil and is pollinated by insects. It can grow for many years. To study the population structure trends and genetic diversity of 150 oil palm lines from Indian indigenous collections. A total of 54 SSR loci were used to amplify DNA from 150 oil palm samples. The amplification results revealed a considerable degree of genetic heterogeneity among oil palm individuals based on 54 polymorphic microsatellite loci. The average PIC value for all polymorphic loci across oil palm genotypes was 0.44, ranging from 0.19 to 0.78. However, the mixing of different genotypes was better explained by the SSR markers. Of the 54 SSRs examined in this study, mEgCIR0246, mEgCIR3358, mEgCIR0782, and mEgCIR0779 show a respectably high degree of polymorphism. The mean of Heterozygosity (Ho) was 0.28 and 150 genotypes were split into two main groups using unweighted pair-group technique. The oil palm lines were found to have originated from two separate sources. The SSR method was found to be effective and reliable for measuring the genetic diversity in oil palm. Knowing about genetic diversity and how different populations are structured is very important for managing genetic resources in a way that helps future breeding programs.

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References

Abimbola, L. M., Din, K. A., Nookiah, R., Hassan, M. S., Ngah, C. W. Z. C. W., & Sukaimi, J. (2016). Genetic variability for yield, yield components and fatty acid traits in oil palm (Elaeis guineensis Jacq.) germplasm using multivariate tools. International Journal of Agriculture, Forestry and Plantation, 2, 219-226.

Babu, B. K, Mary Rani K. L., Sahu, S., Mathur, R. K., Naveen Kumar, P., Ravichandran, G., Anitha, P., & Bhagya, H. P. (2019). Development and validation of whole genome-wide and genic microsatellite markers in oil palm (Elaeis guineensis Jacq.): First microsatellite database (OpSatdb). Scientific reports, 9, 1899. https://doi.org/10.1038/s41598-018-37737-7

Babu, B. K, Mathur, R. K., Ravichandran, G., Anitha, P., Bhagya, H. P., & Venu, M. V. B. (2023). A novel QTL linked toasparagine synthetase gene forstem height increment in oil palm (E. guineensis Jacq.) identifed and validated through integrated genomic approaches. Euphytica, 219, 73. https://doi.org/10.1007/s10681-023-03201-5

Babu, B. K., Dinesh, P., Agrawal, P. K., Sood, S., Chandrashekara, C., Bhatt, J. C., & Kumar, A. (2014). Comparative genomics and association mapping approaches for blast resistant genes in finger millet using SSRs. Plos One, 9(6), e99182. https://doi.org/10.1371/journal.pone.0099182

Babu, B. K., Mathur, R. K., Venu, M. V. B., Sandip, S., Ravichandran, G., Anita, P., & Bhagya, H. P. (2021). Genome-wide association study (GWAS) of major QTLs for bunch and oil yield-related traits in Elaeis guineensis L. Plant Science, 305,110810. https://doi.org/10.1016/j.plantsci.2020.110810

Bakoumé, C., Wickneswari, R., Siju, S., Rajanaidu, N., Kushairi, A., & Billotte, N. (2015). Genetic diversity of the world’s largest oil palm (Elaeis guineensis Jacq.) field gene bank accessions using microsatellite markers. Genetic Resources and Crop Evolution, 62, 349-360. https://doi.org/10.1007/s10722-014-0156-8

Barcelos, E., Amblard, P., Berthaud, J., & Seguin, M. (2000). The genetic diversity of the American oil palm (Elaeis oleifera Kunth.) Corte’s revealed by nuclear RFLP markers. In International symposium on oil palm genetic resources and utilisation, Kuala Lumpur. Proceedings Kuala Lumpur. The Malaysian Palm Oil Board, 173-192.

Bhagya, H. P., Babu B. K., Naika, M. B. N., Mathur, R. K., Gangadharappa, P. M., Satisha, D., & Naik, R. B. (2018). Identification and utilization of polymorphic SSR markers for genetic diversity studies in oil palm. International Journal of Current Microbiology and Applied Sciences, 7(4), 333-341. https://doi.org/10.20546/ijcmas.2018.704.038

Bhagya, H. P., Babu B. K., Gangadharappa P. M., Naika, M. B. N., Satish, D., & Mathur, R. K. (2020). Identification of QTLs in oil palm (Elaeis guineensisJacq.) using SSR markers through association mapping. Journal of Genetics, 99, 19. https://doi.org/10.1007/s12041-020-1180-4

Bhagya, H. P., Babu, B. K., Mathur, R. K., Ramajayam, D., Ravichandran, G., & Anitha, P. (2024). Oil palm (Elaeis guineensis Jacq.) germplasm genome-wide association analysis for the oil yield traits utilising microsatellite markers. Industrial Crops and Products, 218, 118934. https://doi.org/10.1016/j.indcrop.2024.118934

Bhagya, H. P., Mathur, R. K., Ravichandran, G., Ramajayam, D., Kalyana Babu, B., Anitha, P., Murugesan, P., Sunil Kumar, K., Somasundaram, G., & Rahana, S. N. (2021). Identification and selection of elite oil palm (Elaeis guineensis Jacq.) genotypes for utilisation in a breeding program. Journal of Plantation Crops, 49(3), 162-167. https://doi.org/10.25081/jpc.2021.v49.i3.7449

Botstein, D., White, R. L., Skolnick, M., & Davis, R. W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32(3), 314-331.

Cochard, B., Adon, B., Rekima, S., Billotte, N, de Chenon, R D., Koutou, A., Nouy, B., Omore, A., Purba, A. R., Glaszmann, J.-C., & Noyer, J.-L. (2009). Geographic and genetic structure of African oil palm diversity suggests new approaches to breeding. Tree Genetics & Genomes, 5, 493-504. https://doi.org/10.1007/s11295-009-0203-3

Corley, R. H. V., & Tinker, P. B. (2003). The Oil palm. (4th ed.). Blackwell Science Ltd. https://doi.org/10.1002/9780470750971

Hardon, J. J. (1970). Inbreeding in populations of the oil palm (Elaeis guineensis Jacq.) and its effects on selection. Oléagineux, 25, 449-456.

Hayati, A., Wickneswari, R., Maizura, I., & Rajanaidu, N. (2004). Genetic diversity of oil palm (Elaeis guineensis Jacq.) germplasm collections from Africa: implications for improvement and conservation of genetic resources. Theoretical and Applied Genetics, 108, 1274-1284. https://doi.org/10.1007/s00122-003-1545-0

Heckenberger, M., Bohn, M, Ziegle, J. S., Joe, L. K., Hauser, J. D., Hutton, M., & Melchinger, A. E. (2002). Variation of DNA fingerprints among accessions within maize inbred lines and implications for identification of essentially derived varieties. Molecular Breeding, 10, 181-191. https://doi.org/10.1023/A:1020539330957.

Henson, I. E., & Harun, M. H. (2005). The influence of climatic conditions on gas and energy exchanges above a young oil palm stand in north Kedah, Malaysia. Journal of Oil Palm Research, 17, 73-91.

Hill, W. G., & Mackay, T. F. C. (2004). D. S. Falconer and Introduction to Quantitative Genetics. Genetics, 167(4), 1529-1536. https://doi.org/10.1093/genetics/167.4.1529

Jalani, B. S. (2012). Malaysian oil palm industry: contribution, challenges and future prospects. Nilai, Negeri Sembilan, Malaysia, University Sains Islam Malaysia.

Lanes, É, C. M., Motoike, S. Y., Kuki, K. N., Nick, C., & Freitas, R. D. (2015). Molecular characterization and population structure of the Macaw Palm, Acrocomia aculeata (Arecaceae), Ex Situ germplasm collection using microsatellite markers. Journal of Heredity, 106(1), 102-112. https://doi.org/10.1093/jhered/esu073

Liu, K., & Muse, S. V. (2005). Power Marker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 21, 2128-2129. https://doi.org/10.1093/bioinformatics/bti282

Maizura, I., Rajanaidu, N., Zakri, A. H., & Cheah, S. (2006). Assessment of genetic diversity in oil palm (Elaeis guineensis Jacq.) using Restriction Fragment Length Polymorphism (RFLP). Genetic Resources and Crop Evolution, 53, 187-195. https://doi.org/10.1007/s10722-004-4004-0

Murray, M. G., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8(19), 4321-4326. https://doi.org/10.1093/nar/8.19.4321

Murthy, B. R., & Arunachalam, V. (1966). The nature of genetic divergence in relation to breeding system in crop plants. Indian Journal of Genetics and Plant Breeding, 26, 188-198.

Odong, T. L., van Heerwaarden, J., Jansen, J., van Hintum, T. J. L., & van Eeuwijk, F. A. (2011). Determination of genetic structure of germplasm collections are traditional hierarchial clustering methods appropriate for molecular marker data. Theoretical and Applied Genetics, 123, 195-205. https://doi.org/10.1007/s00122-011-1576-x

Okoye, M. N., Uguru, M. I., Bakoume, C., Singh, R., & Okwuagwu, C. O. (2016). Assesment of genetic diversity of NIFOR oil palm main breeding parent genotypes using microsatellite markers. American Journal of Plant Sciences, 7(1), 218-237. https://doi.org/10.4236/ajps.2016.71022

Ong, P. W., Maizura, I., Abdullah, N. A. P., Rafii, M. Y., Ooi, L. C. L., Low, E. T. L. & Singh, R. (2015). Development of SNP markers and their application for genetic diversity analysis in the oil palm (Elaeis guineensis). Genetics and Molecular Research, 14(4), 12205-12216.

Rajanaidu, N. (1994). PORIM oil palm genebank: collection, evaluation, utilization and conservation of oil palm genetic resources. Palm Oil Research Institute of Malaysia. http://myagric.upm.edu.my/id/eprint/9403

Sapey, E., Adusei-Fosu, K., Darkwah, D. O., & Agyei-Dwarko, D. (2017). Multivariate analysis of bunch yield and vegetative traits of oil palm germplasm conserved at Oil Palm Research Institute (OPRI) -Ghana. International Journal of Plant Breeding and Crop Science, 4(2), 231-236.

Singh, R., Ong-Abdullah, M., Low, E.-T. L., Manaf, M. A. A., Rosli, R., Nookiah, R., Ooi, L. C.-L., Ooi, S.-E., Chan, K.-L., Halim, M. A., Aziz, N., Nagappan, J., Bacher, B., Lakey, N., Smith, S. W., He, D., Hogan, M., Budiman, M. A., Lee, E. K., ... Sambanthamurthi, R. (2013) Oil palm genome sequence reveals divergence of interfertile species in old and new Worlds. Nature, 500, 335-339. https://doi.org/10.1038/nature12309

Singh, R., Zaki, N. M., Ting, N.-C., Rosli, R., Tan, S.-G., Low, E.-T. L., Ithnin, M., & Cheah, S.-C. (2008). Exploiting an oil palm EST database for the development of gene-derived SSR markers and their expoitation for assessment of genetic diversity. Biologia, 63, 227-235. https://doi.org/10.2478/s11756-008-0041-z

Ting, N.-C., Noorhariza, M. Z., Rozana, R., Low, E.-T. L., Ithnin, M., Cheah, S.-C., Tan, S.-G., & Singh, R. (2010). SSR mining in oil palm EST database: application in oil palm germplasm diversity studies. Journal of Genetics, 89, 135-145. https://doi.org/10.1007/s12041-010-0053-7

Ting, N.-C., Yaakub, Z., Kamaruddin, K., Mayes, S., Massawe, F., Sambanthamurthi, R., Jansen, J., Low, L. E. T., Ithnin, M., Kushairi, A., Arulandoo, X., Rosli, R., Chan, K.-L., Amiruddin, N., Sritharan, S., Lim, C. C., Nookiah, R., Amiruddin, M. D., & Singh, R. (2016). Fine-mapping and cross validation of QTLs linked to fatty acid composition in multiple independent interspecific crosses of oil palm. BMC Genomics, 17, 289. https://doi.org/10.1186/s12864-016-2607-4

Xu, W., John Martin, J. J., Li, X., Liu, X., Zhang, R., Hou, M., Cao, H., & Cheng, S. (2024). Unveiling the secrets of oil palm genetics: A look into omics research. International Journal of Molecular Sciences, 25(16), 8625. https://doi.org/10.3390/ijms25168625

Zaki, N. M., Singh, R., Rosli, R., & Ismail, I. (2012). Elaeis oleifera genomic-SSR markers: exploitation in oil palm germplasm diversity and cross-amplification in Arecaceae. International Journal of Molecular Sciences, 13(4), 4069-4088. https://doi.org/10.3390/ijms13044069

Zhou, L., Xiao, Y., Xia, W., & Yang, Y. (2015). Analysis of genetic diversity and population structure of oil palm (Elaeis guineensis) from China and Malaysia based on species-specific simple sequence repeat markers. Genetics and Molecular Research, 14(4), 16247-16254.

Published

11-09-2026

How to Cite

Bhagya, H. P., & Babu, B. K. (2026). Microsatellite-inferred genetic architecture of oil palm germplasm. Journal of Plantation Crops, 54(1), 16–24. https://doi.org/10.25081/jpc.2026.v54.i1.10079

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Research Articles