Optimizing suitable solvent for phenylpropanoid extraction and antioxidant activities in Agastache rugosa hairy roots

Authors

  • Bao Van Nguyen Department of Smart Agriculture Systems, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea
  • Jinsu Lim Department of Bio-AI Convergence, Chungnam National University, 99 Daehak-ro, Daejeon 34134, Republic of Korea
  • Kihyun Kim Department of Crop Science, Chungnam National University, 99 Daehak-ro, Daejeon 34134, Republic of Korea
  • Hyewon Seo Department of Crop Science, Chungnam National University, 99 Daehak-ro, Daejeon 34134, Republic of Korea
  • Ramaraj Sathasivam Department of Crop Science, Chungnam National University, 99 Daehak-ro, Daejeon 34134, Republic of Korea
  • Jong Seok Park Department of Bio-AI Convergence, Chungnam National University, 99 Daehak-ro, Daejeon 34134, Republic of Korea, Department of Horticultural Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea
  • Jae Kwang Kim Division of Life Sciences and Convergence Research Center for Insect Vectors, Incheon National University, Incheon 22012, Republic of Korea
  • Sang Un Park Department of Smart Agriculture Systems, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea, Department of Bio-AI Convergence, Chungnam National University, 99 Daehak-ro, Daejeon 34134, Republic of Korea, Department of Crop Science, Chungnam National University, 99 Daehak-ro, Daejeon 34134, Republic of Korea,

DOI:

https://doi.org/10.25081/jp.2024.v16.8894

Keywords:

Agastache rugosa, Extraction of different solvents, Antioxidants, Phenolics, Flavonoids, Phenylpropanoids

Abstract

Agrobacterium-mediated hairy roots (HRs) can induce genetic stability, rapid growth, and the synthesis of bioactive compounds in plant roots. Agastache rugosa is otherwise called Korea Mint and several studies have reported that this plant has been used for the treatment of various diseases due to the presence of a variety of bioactive compounds. A. rugosa HRs are rich in secondary metabolites than the seedling roots, and the HRs extract might be more useful in pharmacology, especially in cosmetology. This study aimed to select the suitable solvent for the extraction of phenylpropanoid compounds, total phenolic (TP), flavonoid (TF), and antioxidant activities (DPPH, ABTS scavenging activity, and reducing power assay). In this study, we extracted the A. rugosa HRs with three different extracts of solvent (water, MeOH, and EtOH) of A. rugosa HRs and analyzed the phenylpropanoid compounds, TP, TF, and antioxidant activities. The result showed that 70% MeOH extracts showed the highest activities in all assays, followed by the 70% EtOH, and water extracts. In addition, 70% of MeOH extracts showed the highest TP and TF (46.14±0.25 GAE mg/g DW and 65.46±1.41 QE mg/g DW, respectively) contents, which was 1.96- and 1.76- times higher than that of the water extracts. The phenylpropanoids in A. rugosa HR extracts were identified by using HPLC, and the results showed that 70% MeOH and EtOH showed the highest contents. Regarding these results, we can conclude that 70% MeOH is the optimal solvent to extract the A. rugosa HRs for the highest phenylpropanoid, TP, TF content, and antioxidant activities. This study might be useful for producing useful compounds at an industrial scale.

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Published

26-04-2024

How to Cite

Nguyen, B. V., Lim, J., Kim, K., Seo, H., Sathasivam, R., Park, J. S., Kim, J. K., & Park, S. U. (2024). Optimizing suitable solvent for phenylpropanoid extraction and antioxidant activities in Agastache rugosa hairy roots. Journal of Phytology, 16, 87–93. https://doi.org/10.25081/jp.2024.v16.8894

Issue

Volume 16, 2024

Section

Articles

Copyright (c) 2024 Bao Van Nguyen, Jinsu Lim, Kihyun Kim, Hyewon Seo, Ramaraj Sathasivam, Jong Seok Park, Jae Kwang Kim, Sang Un Park

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