Influence of pectin on phenylpropanoid accumulation in buckwheat (Fagopyrum esculentum) sprout
DOI:
https://doi.org/10.25081/jp.2023.v15.8196Keywords:
Pectin, phenylpropanoid, common buckwheat, Fagopyrum esculentum MoenchAbstract
Buckwheat (Fagopyrum esculentum Monech) contains several secondary metabolites like phenolic chemicals. Pectin has been demonstrated to be an efficient elicitor from the biotic group for triggering the defensive response, which enhances the production of secondary metabolites. In this study, the effect of pectin on the growth of buckwheat sprouts and the production of phenylpropanoid compounds in common buckwheat sprouts was investigated by using high-performance liquid chromatography (HPLC). Pectin treatments of 0, 2, 4, 6, and 8 mg/L were administered on buckwheat sprouts for ten days to assess the growth characteristics and optimum concentrations. In comparison to the control treatment, 2 mg/L pectin enhances the shoot length by 24%. But when pectin concentration continued to rise, a tendency toward shorter shoots was seen. Pectin treatment decreased the fresh weight of the sprout as compared to the control treatment. The phenylpropanoid accumulation in buckwheat sprouts varied depending on the amount of pectin utilized. Pectin treatment at 6 mg/L resulted in a 15.10% increase in total phenylpropanoid accumulation. The findings of this study indicate that pectin is a possible elicitor, however, more research on how pectin affects the buildup of phenylpropanoids in buckwheat sprouts would be more intriguing to examine the implications of this work.
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Artés-Hernández, F., Castillejo, N., & Martínez-Zamora, L. (2022). UV and visible spectrum LED lighting as abiotic elicitors of bioactive compounds in sprouts, microgreens, and baby leaves. A comprehensive review including their mode of action. Foods, 11(3), 265. https://doi.org/10.3390/foods11030265
Blamey, F. P. C. (2003). A role for pectin in the control of cell expansion. Soil Science and Plant Nutrition, 49(6), 775-783. https://doi.org/10.1080/00380768.2003.10410339
Cai, Z., Kastell, A., Mewis, I., Knorr, D., & Smetanska, I. (2012). Polysaccharide elicitors enhance anthocyanin and phenolic acid accumulation in cell suspension cultures of Vitis vinifera. Plant Cell, Tissue and Organ Culture, 108, 401-409. https://doi.org/10.1007/s11240-011-0051-3
Chandel, V., Biswas, D., Roy, S., Vaidya, D., Verma, A., & Gupta, A. (2022). Current advancements in pectin: Extraction, properties and multifunctional applications. Foods, 11(17), 2683. https://doi.org/10.3390/foods11172683
Chen, Y., Li, W., Turner, J. A., & Anderson, C. T. (2021). PECTATE LYASE LIKE12 patterns the guard cell wall to coordinate turgor pressure and wall mechanics for proper stomatal function in Arabidopsis. The Plant Cell, 33(9), 3134-3150. https://doi.org/10.1093/plcell/koab161
Clemens, S., & Weber, M. (2016). The essential role of coumarin secretion for Fe acquisition from alkaline soil. Plant Signalling & Behavior, 11(2), e1114107. https://doi.org/10.1080/15592324.2015.1114197
Cuong, D. M., Ha, T. W., Park, C. H., Kim, N. S., Yeo, H. J., Chun, S. W., Kim, C., & Park, S. U. (2019). Effects of LED lights on expression of genes involved in phenylpropanoid biosynthesis and accumulation of phenylpropanoids in wheat sprout. Agronomy, 9(6), 307. https://doi.org/10.3390/agronomy9060307
Dong, N. Q., & Lin, H. X. (2021). Contribution of phenylpropanoid metabolism to plant development and plant-environment interactions. Journal of Integrative Plant Biology, 63(1), 180-209. https://doi.org/10.1111/jipb.13054
Dornenburg, H., & Knorr, D. (1995). Strategies for the improvement of secondary metabolite production in plant cell cultures. Enzyme and Microbial Technology, 17(8), 674-684. https://doi.org/10.1016/0141-0229(94)00108-4
Ebert, A. W. (2022). Sprouts and Microgreens-Novel Food Sources for Healthy Diets. Plants, 11(4), 571. https://doi.org/10.3390/plants11040571
Grover, S., Shinde, S., Puri, H., Palmer, N., Sarath, G., Sattler, S. E., & Louis, J. (2022). Dynamic regulation of phenylpropanoid pathway metabolites in modulating sorghum defense against fall armyworm. Frontiers in Plant Science, 13, 1019266. https://doi.org/10.3389/fpls.2022.1019266
Huda, M. N., Lu, S., Jahan, T., Ding, M., Jha, R., Zhang, K., Zhang, W., Georgiev, M. I., Park, S. U., & Zhou, M. (2021). Treasure from garden: Bioactive compounds of buckwheat. Food Chemistry, 335, 127653. https://doi.org/10.1016/j.foodchem.2020.127653
Iwashina, T. (2000). The structure and distribution of the flavonoids in plants. Journal of Plant Research, 113(3), 287-299. https://doi.org/10.1007/PL00013940
Kreft, M. (2016). Buckwheat phenolic metabolites in health and disease. Nutrition Research Reviews, 29(1), 30-39. https://doi.org/10.1017/s0954422415000190
Mansur, A. R., Lee, S. G., Lee, B.-H., Han, S. G., Choi, S.-W., Song, W.-J., & Nam, T. G. (2022). Phenolic compounds in common buckwheat sprouts: composition, isolation, analysis and bioactivities. Food Science and Biotechnology, 31, 935-956. https://doi.org/10.1007/s10068-022-01056-5
Matsui, K., & Yasui, Y. (2020). Genetic and genomic research for the development of an efficient breeding system in heterostylous self-incompatible common buckwheat (Fagopyrum esculentum). Theoretical and Applied Genetics, 133, 1641-1653. https://doi.org/10.1007/s00122-020-03572-6
Park, C. H., Yeo, H. J., Park, Y. J., Morgan, A. M. A., Arasu, M. V., Al-Dhabi, N. A., & Park, S. U. (2017). Influence of indole-3-acetic acid and gibberellic acid on phenylpropanoid accumulation in common buckwheat (Fagopyrum esculentum Moench) sprouts. Molecules, 22(3), 374. https://doi.org/10.3390/molecules22030374
Peaucelle, A., Louvet, R., Johansen, J. N., Höfte, H., Laufs, P., Pelloux, J., & Mouille, G. (2008). Arabidopsis phyllotaxis is controlled by the methyl-esterification status of cell-wall pectins. Current Biology, 18(24), 1943-1948. https://doi.org/10.1016/j.cub.2008.10.065
Pratyusha, D. S., & Sarada, D. V. L. (2022). MYB transcription factors-master regulators of phenylpropanoid biosynthesis and diverse developmental and stress responses. Plant Cell Reports, 41, 2245-2260. https://doi.org/10.1007/s00299-022-02927-1
Ramaroson, M. L., Koutouan, C., Helesbeux, J.-J., Le Clerc, V., Hamama, L., Geoffriau, E., & Briard, M. (2022). Role of phenylpropanoids and flavonoids in plant resistance to pests and diseases. Molecules, 27(23), 8371. https://doi.org/10.3390/molecules27238371
Sathasivam, R., Kim, M. C., Yeo, H. J., Nguyen, B. V., Sohn, S. I., Park, S. U., & Kim, J. (2021). Accumulation of phenolic compounds and glucosinolates in sprouts of pale green and purple kohlrabi (Brassica oleracea var. gongylodes) under light and dark conditions. Agronomy, 11(10), 1939. https://doi.org/10.3390/agronomy11101939
Shakya, P., Marslin, G., Siram, K., Beerhues, L., & Franklin, G. (2019). Elicitation as a tool to improve the profiles of high-value secondary metabolites and pharmacological properties of Hypericum perforatum. The Journal of Pharmacy and Pharmacology, 71(1), 70-82. https://doi.org/10.1111/jphp.12743
Ullah, M. A., Gul, F. Z., Khan, T., Bajwa, M. N., Drouet, S., Tungmunnithum, D., Giglioli-Guivarc'h, N., Liu, C., Hano, C., & Abbasi, B. H. (2021). Differential induction of antioxidant and anti-inflammatory phytochemicals in agitated micro-shoot cultures of Ajuga integrifolia Buch. Ham. ex D.Don with biotic elicitors. AMB Express, 11, 137. https://doi.org/10.1186/s13568-021-01297-3
Wiktorowska, E., Długosz, M., & Janiszowska, W. (2010). Significant enhancement of oleanolic acid accumulation by biotic elicitors in cell suspension cultures of Calendula officinalis L. Enzyme and Microbial Technology, 46(1), 14-20. https://doi.org/10.1016/j.enzmictec.2009.09.002
Wolf, S., & Greiner, S. (2012). Growth control by cell wall pectins. Protoplasma, 249, 169-175. https://doi.org/10.1007/s00709-011-0371-5
Zheng, F., Chen, L., Zhang, P., Zhou, J., Lu, X., & Tian, W. (2020). Carbohydrate polymers exhibit great potential as effective elicitors in organic agriculture: a review. Carbohydrate Polymers, 230, 115637. https://doi.org/10.1016/j.carbpol.2019.115637
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Copyright (c) 2023 Chang Ha Park, Hyeon Ji Yeo, Minsol Choi, Ramaraj Sathasivam, Md. Romij Uddin, Meenakshi Sundaram Muthuraman, Haenghoon Kim, Sang Un Park
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