Expression analysis of diosgenin pathway genes and diosgenin accumulation in fenugreek sprouts after exposure to copper sulfate
DOI:
https://doi.org/10.25081/jp.2022.v14.7769Keywords:
CuSO4, Diosgenin, Fenugreek sprouts, Gene expressionAbstract
Trigonella foenum-graecum L. is an annual herb belonging to the family Fabaceae commonly called Fenugreek. It is rich in various secondary metabolites such as alkaloids, flavonoids, phenolic compounds, and steroidal saponins. In recent years, diosgenin has much attention in the cosmetic, functional food, and pharmaceutical industries. In this study we aimed to examine the effect of different concentrations of copper sulfate (CuSO4) on growth, diosgenin biosynthetic (DB) gene expression, and diosgenin accumulation in T. foenum-graecum sprouts. Results showed that the seed germination, fresh weight, shoot length, and root length were gradually decreased with increasing the CuSO4 concentrations. In contrast, the expression level of DBGs i.e., TfSQS, TfSQLE, TfCAS, and TfSTRL were gradually upregulated with increasing the CuSO4 concentrations. Among all those tested concentrations, the expression levels of all those genes were significantly higher in 0.5 mM CuSO4 treated sprouts. The highest expression level was obtained in the TfCAS gene, which was 3.25-fold higher than the unexposed sprouts. The diosgenin content was significantly influenced in the CuSO4 exposed sprouts. The highest diosgenin content was achieved in the 5.0 mM followed by 1.0, 10.0, and 0.5 mM CuSO4 exposed concentrations, with a reduction of 41%, 39%, 36%, and 35%, respectively. From these results, it is shown that exposure of fenugreek sprout to CuSO4 is one of the suitable strategies to enhance the accumulation of diosgenin content.
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Arif, N., Yadav, V., Singh, S., Singh, S., Ahmad, P., Mishra, R. K., Sharma, S., Tripathi, D. K., Dubey, N., & Chauhan, D. K. (2016). Influence of high and low levels of plant-beneficial heavy metal ions on plant growth and development. Frontiers in Environmental Science, 4, 69. https://doi.org/10.3389/fenvs.2016.00069
Cambrollé, J., Mancilla-Leytón, J., Muñoz-Vallés, S., Figueroa-Luque, E., Luque, T., & Figueroa, M. (2013). Effects of copper sulfate on growth and physiological responses of Limoniastrum monopetalum. Environmental Science and Pollution Research, 20, 8839-8847. https://doi.org/10.1007/s11356-013-1833-4
Chen, C. -T., Wang, Z. -H., Hsu, C. -C., Lin, H. -H., & Chen, J. -H. (2015). In vivo protective effects of diosgenin against doxorubicin-induced cardiotoxicity. Nutrients, 7(6), 4938-4954. https://doi.org/10.3390/nu7064938
Chipeng, F. K., Hermans, C., Colinet, G., Faucon, M. -P., Ngongo, M., Meerts, P., & Verbruggen, N. (2010). Copper tolerance in the cuprophyte Haumaniastrum katangense (S. Moore) PA Duvign. & Plancke. Plant and Soil, 328(1), 235-244. https://doi.org/10.1007/s11104-009-0105-z
Guo, R., Ebenezer, V., & Ki, J. -S. (2012). Transcriptional responses of heat shock protein 70 (Hsp70) to thermal, bisphenol A, and copper stresses in the dinoflagellate Prorocentrum minimum. Chemosphere, 89(5), 512-520. https://doi.org/10.1016/j.chemosphere.2012.05.014
Guo, R., Lee, M. -A., & Ki, J. -S. (2013). Different transcriptional responses of heat shock protein 70/90 in the marine diatom Ditylum brightwellii exposed to metal compounds and endocrine-disrupting chemicals. Chemosphere, 92(5), 535-543. https://doi.org/10.1016/j.chemosphere.2013.03.052
Kumar, V., Desai, D., & Shriram, V. (2014). Hairy root induction in Helicteres isora L. and production of diosgenin in hairy roots. Natural Products and Bioprospecting, 4, 107-112. https://doi.org/10.1007/s13659-014-0011-9
Laila, O., & Murtaza, I. (2015). Fenugreek: a treasure of bioactive compounds with promising antidiabetic potential. International Journal of Food and Nutritional Sciences, 4, 149-157.
Lequeux, H., Hermans, C., Lutts, S., & Verbruggen, N. (2010). Response to copper excess in Arabidopsis thaliana: impact on the root system architecture, hormone distribution, lignin accumulation and mineral profile. Plant Physiology and Biochemistry, 48(8), 673-682. https://doi.org/10.1016/j.plaphy.2010.05.005
Liu, K., Zhao, W., Gao, X., Huang, F., Kou, J., & Liu, B. (2012). Diosgenin ameliorates palmitate-induced endothelial dysfunction and insulin resistance via blocking IKKβ and IRS-1 pathways. Atherosclerosis, 223(2), 350-358. https://doi.org/10.1016/j.atherosclerosis.2012.06.012
Mischitelli, M., Jemaà, M., Almasry, M., Faggio, C., & Lang, F. (2016). Ca2+ entry, oxidative stress, ceramide and suicidal erythrocyte death following diosgenin treatment. Cellular Physiology and Biochemistry, 39(4), 1626-1637. https://doi.org/10.1159/000447864
Mourato, M. P., Moreira, I. N., Leitão, I., Pinto, F. R., Sales, J. R., & Martins, L. L. (2015). Effect of heavy metals in plants of the genus Brassica. International Journal of Molecular Sciences, 16(8), 17975-17998. https://doi.org/10.3390/ijms160817975
Nagajyoti, P., Lee, K., & Sreekanth, T. (2010). Heavy metals, occurrence and toxicity for plants: a review. Environmental Chemistry Letters, 8, 199-216. https://doi.org/10.1007/s10311-010-0297-8
Narula, A., Kumar, S., & Srivastava, P. (2005). Abiotic metal stress enhances diosgenin yield in Dioscorea bulbifera L. cultures. Plant Cell Reports, 24, 250-254. https://doi.org/10.1007/s00299-005-0945-9
Rahimzadeh, P., Hosseini, S., & Dilmaghani, K. (2011). Effects of UV-A and UV-C radiation on some morphological and physiological parameters in Savory (Satureja hortensis L.). Annals of Biological Research, 2(5), 164-171.
Raju, J., & Bird, R. P. (2007). Diosgenin, a naturally occurring furostanol saponin suppresses 3-hydroxy-3-methylglutaryl CoA reductase expression and induces apoptosis in HCT-116 human colon carcinoma cells. Cancer Letters, 255(2), 194-204. https://doi.org/10.1016/j.canlet.2007.04.011
Saghirzadeh Darki, B., Shabani, L., Pourvaez, R., & Ghannadian, M. (2019). Effects of CuSO4 and AgNO3 on artemisinin and phenolic compound in shoot cultures of Artemisia annua L. Journal of Plant Process and Function, 8(31), 1-8.
Sathasivam, R., Guo, R., Wang, H., Lim, W., & Ki, J. -S. (2018). Expressed sequence tag library of the marine green alga Tetraselmis suecica: a focus on stress-related genes for marine pollution. Journal of Applied Phycology, 30, 2387-2402. https://doi.org/10.1007/s10811-018-1445-y
Sathasivam, R., & Ki, J. -S., (2019a). Differential transcriptional responses of carotenoid biosynthesis genes in the marine green alga Tetraselmis suecica exposed to redox and non-redox active metals. Molecular Biology Reports, 46, 1167-1179. https://doi.org/10.1007/s11033-018-04583-9
Sathasivam, R., & Ki, J. -S. (2019b). Heat shock protein genes in the green alga Tetraselmis suecica and their role against redox and non-redox active metals. European Journal of Protistology, 69, 37-51. https://doi.org/10.1016/j.ejop.2019.01.006
Schutzendubel, A. (2002). Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. Journal of Experimental Botany, 53(372), 1351-1365. https://doi.org/10.1093/jexbot/53.372.1351
Shah, F. U. R., Ahmad, N., Masood, K. R., & Peralta-Videa, J. R. (2010). Heavy metal toxicity in plants. In M. Ashraf, M. Ozturk, M. S. A. Ahmad (Eds.), Plant Adaptation and Phytoremediation (pp. 71-97) Switzerland: Springer Dordrecht. https://doi.org/10.1007/978-90-481-9370-7
Siedlecka, A. (1995). Some aspects of interactions between heavy metals and plant mineral nutrients. Acta Societatis Botanicorum Poloniae, 64(3), 265-272. https://doi.org/10.5586/asbp.1995.035
Snehlata, H. S., & Payal, D. R. (2012). Fenugreek (Trigonella foenum-graecum L.): an overview. International Journal of Current Pharmaceutical Review and Research, 2(4), 169-187.
Son, I. S., Kim, J. H., Sohn, H. Y., Son, K. H., Kim, J. -S., & Kwon, C. -S. (2007). Antioxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam (Dioscorea spp.), on high-cholesterol fed rats. Bioscience, Biotechnology, and Biochemistry, 71(12), 3063-3071. https://doi.org/10.1271/bbb.70472
Trejo-Tapia, G., Jimenez-Aparicio, A., Rodriguez-Monroy, M., Jesus-Sanchez, D., & Gutierrez-Lopez, G. (2001). Influence of cobalt and other microelements on the production of betalains and the growth of suspension cultures of Beta vulgaris. Plant Cell, Tissue and Organ Culture, 67, 19-23. https://doi.org/10.1023/A:1011684619614
Venn, A. A., Quinn, J., Jones, R., & Bodnar, A. (2009). P-glycoprotein (multi-xenobiotic resistance) and heat shock protein gene expression in the reef coral Montastraea franksi in response to environmental toxicants. Aquatic Toxicology, 93(4), 188-195. https://doi.org/10.1016/j.aquatox.2009.05.003
Wani, S. A., & Kumar, P. (2016). Fenugreek: A review on its nutraceutical properties and utilization in various food products. Journal of the Saudi Society of Agricultural Sciences, 17(2), 97-106. https://doi.org/10.1016/j.jssas.2016.01.007
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Copyright (c) 2022 Do Yeon Kwon, Ramaraj Sathasivam, Yeon Bok Kim, Haeng-Hoon Kim, Sang Un Park, Byung Bae Park
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