Effect of silver nitrate and putrescine on in vitro shoot organogenesis of Polygonum multiflorum
DOI:
https://doi.org/10.25081/jp.2022.v14.7593Keywords:
Polygonum multiflorum, plant regeneration, silver nitrate, putrescineAbstract
Polygonum multiflorum is a flowering plant that belongs to the family Polygonaceae and it is commonly used for medicinal and ornamental purposes. Few studies have been studied about the regeneration of this species. Therefore, we aimed to develop a suitable protocol for regeneration and subsequent growth of shoots by comparing the silver nitrate (AgNO3) (ethylene inhibitor) and the putrescine (polyamine). Internode explants were cultured on shoot regeneration media (Murashige and Skoog (MS) media containing 2 mg L-1 of 6-benzylaminopurine). To analysis, the effect of AgNO3 and putrescine on shoot regeneration and length, different concentrations (mg L-1) of AgNO3 (0, 1, 5, 7, 10, and 20) and putrescine (0, 10, 30, 50, 100, and 200) were added to the MS media. The result showed that at highest concentration (20 mg L-1) of AgNO3 treatment decrease number of shoots (NOS) (1.4 ± 0.2 mm) and shoot length (9.7 ± 1.6 mm) was observed. Putrescine considerably increased the regeneration efficiency, NOS per explant, and shoot length in all the concentrations when compared to AgNO3 treatment. Among the different concentrations, the highest NOS (2.52 ± 0.2 mm) was obtained in cultures supplemented with 30 mg L-1 putrescine, whereas the further increase in putrescine concentration reduced shoot regeneration. The longest shoots (20.5 ± 1.7 mm) were achieved in cultures supplemented with 200 mg L-1 putrescine. The findings of this study indicate that the addition of putrescine to the media could be suitable for P. multiflorum micropropagation and plant transformation.
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Akasaka-Kennedy, Y., Yoshida, H., & Takahata, Y. (2005). Efficient plant regeneration from leaves of rapeseed (Brassica napus L.): the influence of AgNO3 and genotype. Plant Cell Reports, 24(11), 649-654. https://doi.org/10.1007/s00299-005-0010-8
Al-Shafeay, A. F., Ibrahim, A. S., Nesiem, M. R., & Tawfik, M. S. (2011). Establishment of regeneration and transformation system in Egyptian sesame (Sesamum indicum L.) cv Sohag 1. GM Crops, 2(3), 182-192. https://doi.org/10.4161/gmcr.2.3.18378
Arun, M., Chinnathambi, A., Subramanyam, K., Karthik, S., Sivanandhan, G., Theboral, J., Alharbi, S. A., Kim, C. K., & Ganapathi, A. (2016). Involvement of exogenous polyamines enhances regeneration and Agrobacterium-mediated genetic transformation in half-seeds of soybean. 3 Biotech, 6(2), 1-12. https://doi.org/10.1007%2Fs13205-016-0448-0
Bounda, G. A., & Feng, Y. (2015). Review of clinical studies of Polygonum multiflorum Thunb. and its isolated bioactive compounds. Pharmacognosy Research, 7(3), 225-236. https://doi.org/10.4103/0974-8490.157957
Han, M. N., Lu, J. M., Zhang, G. Y., Yu, J., & Zhao, R. H. (2015). Mechanistic studies on the use of Polygonum multiflorum for the treatment of hair graying. BioMed Research International, 2015, 651048. https://doi.org/10.1155/2015/651048
Kim, H. K., Choi, Y. H., Choi, J. S., Choi, S. U., Kim, Y. S., Lee, K. R., Kim, Y. -K., & Ryu, S. Y. (2008). A new stilbene glucoside gallate from the roots of Polygonum multiflorum. Archives of Pharmacal Research, 31(10), 1225-1229. https://doi.org/10.1007/s12272-001-2100-7
Lee, S. Y., Ahn, S. M., Wang, Z., Choi, Y. W., Shin, H. K., & Choi, B. T. (2017). Neuroprotective effects of 2, 3, 5, 4′-tetrahydoxystilbene-2-O-β-D-glucoside from Polygonum multiflorum against glutamate-induced oxidative toxicity in HT22 cells. Journal of Ethnopharmacology, 195, 64-70. https://doi.org/10.1016/j.jep.2016.12.001
Lei, X., Chen, J., Ren, J., Li, Y., Zhai, J., Mu, W., Zhang, L., Zheng, W., Tian, G., & Shang, H. (2015). Liver damage associated with Polygonum multiflorum Thunb.: a systematic review of case reports and case series. Evidence-Based Complementary and Alternative Medicine, 2015, 459749. https://doi.org/10.1155/2015/459749
Lin, C. L., Hsieh, S. L., Leung, W., Jeng, J. H., Huang, G. C., Lee, C. T., & Wu, C. C. (2016). 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucoside suppresses human colorectal cancer cell metastasis through inhibiting NF-κB activation. International Journal of Oncology, 49(2), 629-638. https://doi.org/10.3892/ijo.2016.3574
Lin, L. C., Nalawade, S. M., Mulabagal, V., Yeh, M. S., & Tsay, H. S. (2003). Micropropagation of Polygonum multiflorum THUNB and quantitative analysis of the anthraquinones emodin and physcion formed in in vitro propagated shoots and plants. Biological and Pharmaceutical Bulletin, 26(10), 1467-1471. https://doi.org/10.1248/bpb.26.1467
Lin, L., Ni, B., Lin, H., Zhang, M., Li, X., Yin, X., Qu, C., & Ni, J. (2015). Traditional usages, botany, phytochemistry, pharmacology and toxicology of Polygonum multiflorum Thunb.: a review. Journal of Ethnopharmacology, 159, 158-183. https://doi.org/10.1016/j.jep.2014.11.009
Ling, S., & Xu, J. W. (2016). Biological activities of 2, 3, 5, 4′-tetrahydroxystilbene-2-O-β-D-glucoside in antiaging and antiaging-related disease treatments. Oxidative Medicine and Cellular Longevity, 2016, 4973239. https://doi.org/10.1155/2016/4973239
Mookkan, M., & Andy, G. (2014). AgNO3 boosted high-frequency shoot regeneration in Vigna mungo (L.) Hepper. Plant Signaling and Behavior, 9(10), e972284. https://doi.org/10.4161/psb.32165
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Park, S. Y., Jin, M. L., Kang, N. J., Park, G., & Choi, Y. W. (2017). Anti-inflammatory effects of novel Polygonum multiflorum compound via inhibiting NF-κB/MAPK and upregulating the Nrf2 pathways in LPS-stimulated microglia. Neuroscience Letters, 651, 43-51. https://doi.org/10.1016/j.neulet.2017.04.057
Park, Y. J., Cheon, G. Y., Song, H. W., Shin, C. S., Ku, Y. G., Kang, N. R., & Heo, B. G. (2016). Mineral composition and physiological activities of methanol extract from the seeds of Persicaria tinctoria. Korean Journal of Plant Resources, 29(1), 32-38. https://doi.org/10.7732/kjpr.2016.29.1.032
Roshanfekrrad, M., Zarghami, R., Hassani, H., Zakizadeh, H., & Salari, A. (2017). Effect of AgNO3 and BAP on root as a novel explant in date palm (Phoenix dactylifera cv. Medjool) somatic embryogenesis. Pakistan Journal of Biological Sciences, 20(1), 20-27. https://doi.org/10.3923/pjbs.2017.20.27
Sextius, P., Betts, R., Benkhalifa, I., Commo, S., Eilstein, J., Massironi, M., Wang, P., Michelet, J.F., Qiu, J., & Tan, X. (2017). Polygonum multiflorum Radix extract protects human foreskin melanocytes from oxidative stress in vitro and potentiates hair follicle pigmentation ex vivo. International Journal of Cosmetic Science, 39(4), 419-425. https://doi.org/10.1111/ics.12391
Shinju, H., Higuchi, M., & Okada, M. (1994). Studies on cultivation of Polygonum multiflorum Thunberg (Part 1) on the methods of vegetative propagation. Natural Medicines, 48(2), 126-130.
Shyamali, S., & Hattori, K. (2007). Effect of polyamines and silver nitrate on the high frequency regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria; sp. asiatica). Pakistan Journal of Biological Sciences, 10(8), 1288-1293. https://doi.org/10.3923/pjbs.2007.1288.1293
Sivanandhan, G., Vasudevan, V., Selvaraj, N., Lim, Y. P., & Ganapathi, A. (2015). L-Dopa production and antioxidant activity in Hybanthus enneaspermus (L.) F. Muell regeneration. Physiology and Molecular Biology of Plants, 21(3), 395-406. https://doi.org/10.1007/s12298-015-0302-6
Sun, Y. N., Cui, L., Li, W., Yan, X. T., Yang, S. Y., Kang, J. I., Kang, H. K., & Kim, Y. H. (2013). Promotion effect of constituents from the root of Polygonum multiflorum on hair growth. Bioorganic and Medicinal Chemistry Letters, 23(17), 4801-4805. https://doi.org/10.1016/j.bmcl.2013.06.098
Tang, W., Li, S., Liu, Y., Wu, J. C., Pan, M. H., Huang, M. T., & Ho, C. T. (2017). Anti‐diabetic activities of cis‐and trans‐2, 3, 5, 4′‐tetrahydroxystilbene 2‐O‐β‐glucopyranoside from Polygonum multiflorum. Molecular Nutrition and Food Research, 61(8), 1600871. https://doi.org/10.1002/mnfr.201600871
Thang, N. D., Diep, P. N., Lien, P. T. H., & Lien, L. T. (2017). Polygonum multiflorum root extract as a potential candidate for treatment of early graying hair. Journal of Advanced Pharmaceutical Technology and Research, 8(1), 8-13. https://doi.org/10.4103/2231-4040.197332
Uliaie, E., Farsi, M., Ghreyazie, B., & Imani, J. (2008). Effects of genotype and AgNO3 on shoot regeneration in winter cultivars of rapeseed (Brassica napus). Pakistan Journal of Biological Sciences, 11(16), 2040-2043. https://doi.org/10.3923/pjbs.2008.2040.2043
Xian, Z., Liu, Y., Xu, W., Duan, F., Guo, Z., & Xiao, H. (2017). The anti-hyperlipidemia effects of raw Polygonum multiflorum extract in vivo. Biological and Pharmaceutical Bulletin, 40(11), 1839-1845. https://doi.org/10.1248/bpb.b17-00218
Yao, S., Li, Y., & Kong, L. (2006). Preparative isolation and purification of chemical constituents from the root of Polygonum multiflorum by high-speed counter-current chromatography. Journal of Chromatography A, 1115(1-2), 64-71. https://doi.org/10.1016/j.chroma.2006.02.071
Yi, T., Leung, K. S., Lu, G. H., Zhang, H., & Chan, K. (2007). Identification and determination of the major constituents in traditional Chinese medicinal plant Polygonum multiflorum thunb by HPLC coupled with PAD and ESI/MS. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 18(3), 181-187. https://doi.org/10.1002/pca.963
Zhu, W., Xue, X., & Zhang, Z. (2016). Ultrasonic-assisted extraction, structure and antitumor activity of polysaccharide from Polygonum multiflorum. International Journal of Biological Macromolecules, 91, 132-142. https://doi.org/10.1016/j.ijbiomac.2016.05.061
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Copyright (c) 2022 Woo Tae Park, Yeon Bok Kim, Ramaraj Sathasivam, Haeng-Hoon Kim, Sang Un Park
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