Identification and characterization of popular rice (Oryza sativa L.) varieties through chemical tests

  • M.S. Nagendra Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore- 641003, Tamil Nadu, India
  • P. Selvaraju Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore- 641003, Tamil Nadu, India
  • R. Jerlin Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore- 641003, Tamil Nadu, India
  • K. Ganesamurthy Department of Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore- 641003, Tamil Nadu, India
  • N. Senthil Department of Plant Molecular Biology and Bioinformatics, Tamil Nadu Agricultural University, Coimbatore- 641003, Tamil Nadu, India

Abstract

Identification and characterization of crop varieties are crucial for ensuring the genetic purity of seeds. The present investigation was carried out to identify suitable chemical methods that are fast, reliable and easy for seed analysts, breeders and seed producers for identification of a variety. Twenty-five popular rice varieties in the seed supply chain of Tamil Nadu were subjected to phenol, modified phenol, NaOH, aroma, gelatinization temperature (alkali spreading value), GA3 and 2,4-D tests. The results of the experiment revealed that phenol and modified phenol tests changed the colour of TKM 9 and TRY 1 variety to brown but no colour change was observed in the variety I.W. Ponni variety. The NaOH test is useful for the identification of TKM 9 variety as it changed the colourless solution to red. GA3 and 2,4-D tests characterized the varieties based on the shoot growth into two and three groups respectively. However, all the variety lacked aroma and exhibited a high gelatinization temperature.

Keywords: Rice, Chemical Test, Phenol Test, Sodium Hydroxide, GA3, Varietal Identification

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How to Cite
Nagendra, M., P. Selvaraju, R. Jerlin, K. Ganesamurthy, and N. Senthil. “Identification and Characterization of Popular Rice (Oryza Sativa L.) Varieties through Chemical Tests”. Journal of Phytology, Vol. 12, Nov. 2020, pp. 82-85, doi:https://doi.org/10.25081/jp.2020.v12.6513.
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