Establishment of callus and cell suspension culture of Sophora alopecuroides Linn. for the production of oxymatrine

  • M. Tsolmon Laboratory of Plant Biotechnology, Institute of General and Experimental Biology, Mongolian Academy of Science, Ulaanbaatar, Mongolia
  • G Oyundari Laboratory of Plant Biotechnology, Institute of General and Experimental Biology, Mongolian Academy of Science, Ulaanbaatar, Mongolia
  • Oyunbileg Yu Laboratory of Plant Biotechnology, Institute of General and Experimental Biology, Mongolian Academy of Science, Ulaanbaatar, Mongolia
  • Kalaiselvi Senthil Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute for Home Science and Higher Education for Women, Coimabtore, Tamilnadu, India

Abstract

Oxymatrine is one of the most important biologically active compounds and is present in Sophora alopecuroides L. The present investigation focuses on the development of an efficient tissue culture method to induce callus and cell suspension culture of S. alopecuroides by studying the effect of jasmonic acid and nitric oxide on cell suspension culture. Callus induction efficiency is high in axenic leaf explants grown in MS medium supplemented with 1.0 mg/L Kinetin (Kin), 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The cell suspension culture was developed using the same callus induction medium without agar. The maximum cell number and dry weight of suspension culture were obtained by the 9th day of incubation. The synthesis of oxymatrine is higher in jasmonic acid and nitric oxide (200 µMJA and 50 µMNO) combination (11.91 µg/g) when compared to the non-elicited control (8.3 µg/g) of callus.

Keywords: Sophora alopecuroides Linn., callus culture, cell suspension culture, oxymatrine, jasmonic acid, nitric oxide.

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How to Cite
Tsolmon, M., G. Oyundari, O. Yu, and K. Senthil. “Establishment of Callus and Cell Suspension Culture of Sophora Alopecuroides Linn. For the Production of Oxymatrine”. Journal of Phytology, Vol. 12, Sept. 2020, pp. 35-39, doi:https://doi.org/10.25081/jp.2020.v12.6308.
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