Identification of Pakistani Isolates of Xanthomonas oryzae pv. oryzae by Insertion Sequence based polymerase chain reaction (IS-PCR)

Authors

  • Shazia Mannan Department of Biosciences, COMSATS Institute of Information Technology Sahiwal, Pakistan
  • Shahid Hameed Crop Diseases Research Programme, National Agricultural Research Center, Park Road, 45500, Islamabad, Pakistan

Keywords:

Xanthomonas oryzae pv. Oryzae, Pakistan, IS-PCR

Abstract

Xanthomonas oryzae pv. oryzae is the causal agent of bacterial blight disease in rice. The pathogen was isolated from infected samples collected after extensive survey of rice growing areas of Pakistan. Afterwards, Insertion sequence based Polymerase chain reaction was applied using the high copy insertion sequence IS1113 based primer for molecular identification of the isolates as well as to differentiate them from Xanthomonas oryzae pv. Oryzicola

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Author Biographies

Shazia Mannan, Department of Biosciences, COMSATS Institute of Information Technology Sahiwal, Pakistan

Department of Biosciences, COMSATS Institute of Information Technology Sahiwal, Pakistan

Shahid Hameed, Crop Diseases Research Programme, National Agricultural Research Center, Park Road, 45500, Islamabad, Pakistan

Crop Diseases Research Programme, National Agricultural Research Center, Park Road, 45500, Islamabad, Pakistan

Published

31-01-2013

How to Cite

Mannan, S., & Hameed, S. (2013). Identification of Pakistani Isolates of Xanthomonas oryzae pv. oryzae by Insertion Sequence based polymerase chain reaction (IS-PCR). Journal of Phytology, 4(5). Retrieved from https://updatepublishing.com/journal/index.php/jp/article/view/2797

Issue

Volume 4, Issue 5, 2012

Section

Research Article