Effects of Carbohydrates on in vitro axillary shoot initiation and multiplication of Bambusa pallida Munro.

Authors

  • Beena D B PG Department of Biotechnology, CMR Institute of Management Studies, Kalyana Nagar, Bangalore – 560 043, India
  • Rathore T S Institute of Wood Science and Technology, 18th Cross, Malleswaram, Bangalore-560 003, India
  • Rao P S Director, Life Science and R&D, Life Science and Engineering Division, Dayananda Sagar College of Engineering, Bangalore, India.

Keywords:

Bambusa pallida, NAA, TDZ, NMBA, Carbohydrates, Axillary shoot

Abstract

The purpose of the current study was to optimize the carbohydrate sources and sucrose concentrations for mass clonal propagation of B. pallida. Sucrose in MS liquid medium consisting additives (ascorbic acid, 50mg/l + citric acid, 25 mg/l + cysteine, 25 mg/l) was found to be the best carbohydrate source for shoot induction and shoot multiplication.  NAA 0.25mg/l in combination with TDZ 0.25mg/l in the medium exhibited high frequency shoot induction and NAA 0.25mg/l with BAP 1.0mg/l helped for further multiplication of quality shoots.  IBA pulse treated shoots were rooted in the MS half strength agar gelled medium fortified with sucrose (2%) and glucose (1%). Rooted plantlets were well established in the green house with more than 95% survivability within four weeks period. 

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Author Biographies

Beena D B, PG Department of Biotechnology, CMR Institute of Management Studies, Kalyana Nagar, Bangalore – 560 043, India

PG Department of Biotechnology, CMR Institute of Management Studies, Kalyana Nagar, Bangalore – 560 043, India

Rathore T S, Institute of Wood Science and Technology, 18th Cross, Malleswaram, Bangalore-560 003, India

Institute of Wood Science and Technology, 18th Cross, Malleswaram, Bangalore-560 003, India

Rao P S, Director, Life Science and R&D, Life Science and Engineering Division, Dayananda Sagar College of Engineering, Bangalore, India.

Director, Life Science and R&D, Life Science and Engineering Division, Dayananda Sagar College of Engineering, Bangalore, India. 

Published

06-02-2013

How to Cite

B, B. D., S, R. T., & S, R. P. (2013). Effects of Carbohydrates on in vitro axillary shoot initiation and multiplication of Bambusa pallida Munro. Journal of Phytology, 4(5). Retrieved from https://updatepublishing.com/journal/index.php/jp/article/view/2786

Issue

Volume 4, Issue 5, 2012

Section

Research Article