Effect of Different Carbon Sources on In Vitro Morphogenetic Response of Patchouli (Pogostemon cablin Benth.)

Abstract

The effect of various carbon sources, sucrose, glucose, fructose, table sugar and sugarcane juice was investigated on in vitro growth and physiology of Pogostemon cablin Benth. The entire morphogenetic pattern was influenced by nature and concentration of carbon source used. The maximum shoot length (4.87±0.41cm) and higher number of multiple shoots (61.43±0.l9) was observed on MS media fortified with 20% sugarcane juice. The maximum fresh weight of shoots was recorded on MS medium containing 2% sucrose (4.89±0.19g). Sugarcane juice at 20% resulted in maximum chlorophyll content (0.81±2.0mg/g tissue). The protein content was maximum on media supplemented with 20% sugarcane juice (18.8±0.24mg/ml) followed by 2% sucrose (18.5±0.25mg/ml). The least content was observed on media supplemented with 3% fructose (12.2±0.32mg/ml). Maximum accumulation of carbohydrate content was observed on MS supplemented with 3% sucrose (18.2±0.30mg/ml) and the least carbohydrate content (11.6±0.53mg/ml) was observed on MS media with 1% glucose. This is the first report on the use of sugarcane juice in tissue culture studies of patchouli.