Stability Studies of Crude Plant Material of Bacopa monnieri and Its Effect on Free Radical Scavenging Activity

Authors

  • Pratibha Srivastava1*, Hema N. Raut1, Hemalata M. Puntambekar1, Anuradha S. Upadhye2 and Anagha C. Desai3

Abstract

Stability study of the crude plant material of Bacopa monnieri has been carried out as per ICH guidelines and its effect on free radical scavenging activity was studied by DPPH, ABTS radical scavenging assays and Deoxyribose degradation assay. The dried whole plant material of B. monnieri was kept in stability chambers at 40°C and 75% relative humidity in case of accelerated studies and at 30°C and 65% relative humidity for long term studies. Real time studies samples were stored at room temperature. Samples were taken out at periodic intervals, extracted in methanol to observe the HPTLC profiles and to study free radical scavenging activity. Results of antioxidant assays indicated that the samples of long term studies showed better antioxidant activity than the samples of accelerated and real time studies.

Key words: Brahmi; accelerated study; long term study; real time study; free radical scavenging activity

Abbreviations: BM: Bacopa monnieri; AS: Accelerated studies; LS: Long term studies; RT Real time studies

Pratibha Srivastava et al. Stability Studies of Crude Plant Material of Bacopa monnieri and Its Effect on Free Radical Scavenging Activity. J Phytol 2/8 (2010) 103-109.

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Author Biography

Pratibha Srivastava1*, Hema N. Raut1, Hemalata M. Puntambekar1, Anuradha S. Upadhye2 and Anagha C. Desai3

1Chemistry Group, Animal Science Division, Agharkar Research Institute, G. G. Agarkar Road,                            Pune-411004, Maharashtra, India2Botany group, Plant Science Division, Agharkar Research Institute, G. G. Agarkar Road,                                      Pune-411004, Maharashtra, India3Drug Analysis, Indian Drug Research Association & Laboratory, 561/B Shivajinagar,                                            Pune-411005, Maharashtra, India

Published

06-12-2010

How to Cite

Hemalata M. Puntambekar1, Anuradha S. Upadhye2 and Anagha C. Desai3, P. S. H. N. R. (2010). Stability Studies of Crude Plant Material of Bacopa monnieri and Its Effect on Free Radical Scavenging Activity. Journal of Phytology, 2(8). Retrieved from https://updatepublishing.com/journal/index.php/jp/article/view/2151

Issue

Section

Phytophysiology