A Simple Staining Method for Increasing Sensitivity in Detection of Alkyl Sulfatase in Native Polyacrylamide Gels

Authors

  • Venkatesh Chaturvedi*, Ashok Kumar

Abstract

SUMMARY

To increase the sensitivity in detection of alkyl sulfatases in Native PAGE gels, we report here a simple staining method based on Coomassie brillent blue R 250. This method facilitates in detection of faint alkyl sulfatase bands which are otherwise difficult to visualize.

Key words: Alkyl sulfatase, Colloidal coomassie, Polyacrylamide gel (PAG)

A Simple Staining Method for Increasing Sensitivity in Detection of Alkyl Sulfatase in Native Polyacrylamide Gels. J Phytol 2/6 (2010) 34-36

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Author Biography

Venkatesh Chaturvedi*, Ashok Kumar

School of Biotechnology, B.H.U, Varanasi – 221005, U.P, India

Published

25-09-2010

How to Cite

Ashok Kumar, V. C. (2010). A Simple Staining Method for Increasing Sensitivity in Detection of Alkyl Sulfatase in Native Polyacrylamide Gels. Journal of Phytology, 2(6). Retrieved from https://updatepublishing.com/journal/index.php/jp/article/view/2142

Issue

Volume 2, Issue 6, 2010

Section

Biotechnology