Somaclonal Variation in Oroxylum indicum (L.) Vent- an Endangered Tree Species

Abstract

Optimum callus formation was observed from apical buds or axillary buds of in vitro grown seedling on Murashige and Skoogs’ (1962) medium supplemented with auxins 0.1-1 mg^-1 of IBA, IAA, NAA and 2,4-D. Morphogenic callus was cultured on media supplemented with additives (CH & AgNO₃ ) with BAP (1 mg^-1). Maximum shoot regeneration  and elongation were achieved upon transferring the callus to medium containing BAP (1 mg^-1) with AgNO₃ (2 mg^-1). Elongated shoots rooted on ½ MSM supplemented with IBA (mg^-1) with AgNO₃ (1 mg^-1). Shoots regenerated from this protocol exhibited somaclonal variation in different characters viz. leaf shape, node size.