Biochemical Analysis of Glycine max Seeds under Different Germinating Periods and Densitometric Analysis of Genistein

Abstract

Comparative analysis of soybean seeds (Glycine max) under different germination period has led to the discovery that germinated seeds have enhanced nutritional quality and can be used for variety of medicinal uses. The protein profiling was done by SDS-PAGE. Carbohydrate quantification showed that that the total sugar content decreases during germination and found to be minimum after boiling. Additionally the starch and α-amylase activity was also checked. Finally, isoflavone profiling of these seeds with different germination periods was done by high performance thin layer chromatography. Furthermore, an important isoflavone, genistein was extracted out and identity of genistein was confirmed by similar HPTLC spectra identical with authentic genistein.

Key words: Diabetes mellitus, Secretagogues, Biguanides, Thiazolidinediones, Incretins, GLP 1 agonist, DPP 4 inhibitors, Bariatric surgery, Antidiabetic plants

Hamid Nawaz Khan et al. Biochemical Analysis of Glycine max Seeds under Different Germinating Periods and Densitometric Analysis of Genistein. J Phytol 2/6 (2010) 83-86.