A Simple and Rapid Method For DNA Extraction From Leaves of Tomato, Tobacco and Rape Seed

Abstract

We have optimized a simple and rapid method for isolation of high quality genomic DNA from leaves of tomato, tobacco and rape seed.  This protocol significantly minimizes time and the use of laboratory materials.  The extracted DNA was suitable for PCR analysis.  This method requires less than 1 mg of leaf tissue and is useful for transgene detection, genetic maps and other DNA based molecular analyses.