Effects of different culture media on growth and lutein production in Tetradesmus dimorphus NUAC202
DOI:
https://doi.org/10.25081/jp.2026.v18.10117Keywords:
Tetradesmus dimorphus, Culture medium, Lutein, β-carotene, Biochemical compoundsAbstract
Microalgae are a source of valuable compounds with broad applications in cosmeceuticals, nutraceuticals, renewable energy, and bioremediation. The present study employs the freshwater microalgae Tetradesmus dimorphus NUAC202 to investigate the effects of different microalgal growth media, like BG-11, Bold’s basal medium (BBM), Bold’s basal medium with 3-fold nitrogen (3N-BBM), Modified Mann & Myers medium (MMM), Modified Bristol’s medium (BMM) and Modified Detmer’s medium (MDM), on its growth, biochemical compounds and photosynthetic pigment production. Lutein and β-carotene content were measured by using high-performance liquid chromatography (HPLC). Growth parameters were significantly improved by MDM and BMM media, whereas maximum accumulation of pigments, especially lutein (2.68 mg/g DW) and β-carotene (0.76 mg/g DW) were observed in BG-11 medium. Moreover, MDM and BG-11-biomass achieved high protein yield (40.64% and 40.49%, respectively). Thus, this study proposes MDM and BG-11 as a suitable media to cultivate T. dimorphus NUAC202 for the co-production of lutein and protein.
Downloads
References
Asker, D., Awad, T. S., Beppu, T., & Ueda, K. (2018). Rapid and selective screening method for isolation and identification of carotenoid-producing bacteria. In C. Barreiro & J.-L. Barredo (Eds.), Microbial Carotenoids (pp. 143-170). Springer. https://doi.org/10.1007/978-1-4939-8742-9
Bermejo, E., Ruiz-Domínguez, M. C., Cuaresma, M., Vaquero, I., Ramos-Merchante, A., Vega, J. M., Vílchez, C., & Garbayo, I. (2018). Production of lutein, and polyunsaturated fatty acids by the acidophilic eukaryotic microalga Coccomyxa onubensis under abiotic stress by salt or ultraviolet light. Journal of Bioscience and Bioengineering, 125(6), 669-675. https://doi.org/10.1016/j.jbiosc.2017.12.025
Cerón, M. C., Campos, I., Sanchez, J. F., Acién, F. G., Molina, E., & Fernandez-Sevilla, J. M. (2008). Recovery of lutein from microalgae biomass: development of a process for Scenedesmus almeriensis biomass. Journal of Agricultural and Food Chemistry, 56(24), 11761-11766. https://doi.org/10.1021/jf8025875
Chang, Y.-R., Lee, D.-J., Chang, J.-S., & Mujumdar, A.-S. (2015). Enhancement of lutein yield from coagulated Chlorella sp. ESP-6 with sodium hypochlorite. Drying Technology, 33(4), 429-433. https://doi.org/10.1080/07373937.2014.970261
Chokshi, K., Pancha, I., Maurya, R., Paliwal, C., Ghosh, T., Ghosh, A., & Mishra, S. (2016). Growth medium standardization and thermotolerance study of the freshwater microalga Acutodesmus dimorphus—a potential strain for biofuel production. Journal of Applied Phycology, 28, 2687-2696. https://doi.org/10.1007/s10811-016-0826-3
Dixit, R., Singh, S., Enamala, M. K., & Patel, A. (2022). Effect of various growth medium on the physiology and de novo lipogenesis of a freshwater microalga Scenedesmus rotundus-MG910488 under autotrophic condition. Clean Technologies, 4(3), 733-751. https://doi.org/10.3390/cleantechnol4030045
Dufossé, L. (2018). Microbial pigments from bacteria, yeasts, fungi, and microalgae for the food and feed industries. In A. M. Grumezescu & A. M. Holban (Eds.), Natural and Artificial Flavoring Agents and Food Dyes (pp. 113-132). Elsevier. https://doi.org/10.1016/B978-0-12-811518-3.00004-1
George, B., Pancha, I., Desai, C., Chokshi, K., Paliwal, C., Ghosh, T., & Mishra, S. (2014) Effects of different media composition, light intensity and photoperiod on morphology and physiology of freshwater microalgae Ankistrodesmus falcatus—a potential strain forbio-fuel production. Bioresource Technology, 171, 367-374. https://doi.org/10.1016/j.biortech.2014.08.086
Gong, M., & Bassi, A. (2016). Carotenoids from microalgae: a review of recent developments. Biotechnology Advances, 34(8), 1396-1412. https://doi.org/10.1016/j.biotechadv.2016.10.005
Hong, S.-J., & Lee, C.-G., 2008. Statistical optimization of culture media for production of phycobiliprotein by Synechocystis sp. PCC 6701. Biotechnology and Bioprocess Engineering, 13, 491-498. https://doi.org/10.1007/s12257-008-0154-9
Kochart, A. G. (1978). Charbohydrate determination by the phenol-sulphuric acid method. In J. A. Hellebust & J. S. Craigie (Eds.). Handbook of Phycological and Biochemical Methods (pp. 95-97). Cambridge University Press.
Lee, N., Ko, S.-R., Ahn, C.-Y., & Oh, H.-M. (2018). Optimized co-production of lipids and carotenoids from Ettlia sp. by regulating stress conditions. Bioresource Technology, 258, 234-239. https://doi.org/10.1016/j.biortech.2018.03.006
Lin, J.-H., Lee, D.-J., & Chang, J.-S. (2015). Lutein production from biomass: marigold flowers versus microalgae. Bioresource Technology, 184, 421-428. https://doi.org/10.1016/j.biortech.2014.09.099
Liu, Y. (2018). Optimization study of biomass and astaxanthin production by Haematococcus pluvialis under minkery wastewater cultures.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 265-275. https://doi.org/10.1016/S0021-9258(19)52451-6
Mandalam, R. K., & Palsson, B. (1998). Elemental balancing of biomass and medium composition enhances growth capacity in high-density Chlorella vulgaris cultures. Biotechnology Bioengineering, 59(5), 605-611. https://doi.org/10.1002/(SICI)1097-0290(19980905)59:5<605::AID-BIT11>3.0.CO;2-8
Ördög, V., Stirk, W. A., Bálint, P., van Staden, J., & Lovász, C. (2012). Changes in lipid,
protein and pigment concentrations in nitrogen-stressed Chlorella minutissima cultures. Journal of Applied Phycology, 24(4), 907-914. https://doi.org/10.1007/s10811-011-9711-2
Piccaglia, R., Marotti, M., & Grandi, S. (1998). Lutein and lutein ester content in different types of Tagetes patula and T. erecta. Industrial Crops and Products, 8(1), 45-51. https://doi.org/10.1016/S0926-6690(97)10005-X
Procházková, G., Brányiková, I., Zachleder, V., & Brányik, T. (2014). Effect of nutrient supply status on biomass composition of eukaryotic green microalgae. Journal of Applied Phycology, 26, 1359-1377. https://doi.org/10.1007/s10811-013-0154-9
Ram, S., Paliwal, C., & Mishra, S. (2019). Growth medium and nitrogen stress sparked biochemical and carotenogenic alterations in Scenedesmus sp. CCNM 1028. Bioresource Technology Reports, 7, 100194. https://doi.org/10.1016/j.biteb.2019.100194
Sanpapao, P., Tawong, W., Pongpadung, P., Ponza, P., & Ponza, S. (2023). Effect of phosphorus and sodium chloride levels on growth performance, carotenoid accumulation and isomerization to 9-cis β-carotene in Thai Dunaliella salina NUAC09. International Aquatic Research, 15, 27-38.
Sathasivam, R., & Ki, J.-S. (2018). A review of the biological activities of microalgal carotenoids and their potential use in healthcare and cosmetic industries. Marine Drugs, 16(1), 26. https://doi.org/10.3390/md16010026
Sathasivam, R., Radhakrishnan, R., Hashem, A., & Abd_Allah, E. F. (2019). Microalgae metabolites: A rich source for food and medicine, 26(4), 709-722. https://doi.org/10.1016/j.sjbs.2017.11.003
Sathasivam, R., Radhakrishnan, R., Kim, J. K., Park, S. U. (2021). An update on biosynthesis and regulation of carotenoids in plants. South African Journal of Botany, 140, 290-302. https://doi.org/10.1016/j.sajb.2020.05.015
Sterner, R. W., & Grover, J. P. (1998). Algal growth in warm temperate reservoirs: kinetic
examination of nitrogen, temperature, light, and other nutrients. Water Research, 32(12), 3539-3548. https://doi.org/10.1016/S0043-1354(98)00165-1
Xie, Y., Ho, S.-H., Chen, C.-N. N., Chen, C.-Y., Ng, I.-S., Jing, K.-J., Chang, J.-S., & Lu, Y. (2013). Phototrophic cultivation of a thermo-tolerant Desmodesmus sp. for lutein production: effects of nitrate concentration, light intensity and fed-batch operation. Bioresource Technology, 144, 435-444. https://doi.org/10.1016/j.biortech.2013.06.064
Published
How to Cite
Issue
Section
Copyright (c) 2026 Piyawat Pongpadung, Nittaya Saninsi, Sunanthakan Khumman, Somrak Rodjaroen, Wittaya Tawong, Supat Ponza, Mahattanee Phinyo, Ramaraj Sathasivam

This work is licensed under a Creative Commons Attribution 4.0 International License.




.