Effects of different culture media on growth and lutein production in Tetradesmus dimorphus NUAC202

Authors

  • Piyawat Pongpadung Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
  • Nittaya Saninsi Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
  • Sunanthakan Khumman Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
  • Somrak Rodjaroen Department of Agriculture, Faculty of Science and Technology, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat 80280, Thailand
  • Wittaya Tawong Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
  • Supat Ponza Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
  • Mahattanee Phinyo Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
  • Ramaraj Sathasivam Department of Integrative Agriculture, College of Agriculture and Veterinary Medicine, United Arab Emirates University, P.O. Box 15551, Al Ain, Abu Dhabi, United Arab Emirates

DOI:

https://doi.org/10.25081/jp.2026.v18.10117

Keywords:

Tetradesmus dimorphus, Culture medium, Lutein, β-carotene, Biochemical compounds

Abstract

Microalgae are a source of valuable compounds with broad applications in cosmeceuticals, nutraceuticals, renewable energy, and bioremediation. The present study employs the freshwater microalgae Tetradesmus dimorphus NUAC202 to investigate the effects of different microalgal growth media, like BG-11, Bold’s basal medium (BBM), Bold’s basal medium with 3-fold nitrogen (3N-BBM), Modified Mann & Myers medium (MMM), Modified Bristol’s medium (BMM) and Modified Detmer’s medium (MDM), on its growth, biochemical compounds and photosynthetic pigment production. Lutein and β-carotene content were measured by using high-performance liquid chromatography (HPLC). Growth parameters were significantly improved by MDM and BMM media, whereas maximum accumulation of pigments, especially lutein (2.68 mg/g DW) and β-carotene (0.76 mg/g DW) were observed in BG-11 medium. Moreover, MDM and BG-11-biomass achieved high protein yield (40.64% and 40.49%, respectively). Thus, this study proposes MDM and BG-11 as a suitable media to cultivate T. dimorphus NUAC202 for the co-production of lutein and protein.

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Published

2026-09-18

How to Cite

Pongpadung, P., Saninsi, N., Khumman, S., Rodjaroen, S., Tawong, W., Ponza, S., Phinyo, M., & Sathasivam, R. (2026). Effects of different culture media on growth and lutein production in Tetradesmus dimorphus NUAC202. Journal of Phytology, 18, 111–115. https://doi.org/10.25081/jp.2026.v18.10117

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