In-Vitro antidiabetic potential of Piliostigma thonningii leaf column chromatographic fractions

Authors

  • Angela Nnenna Ukwuani-Kwaja Department of Biochemistry, Faculty of Life Sciences, Kebbi State University of Science and Technology Aliero, Kebbi State, Nigeria
  • Ibrahim Mohammad Suru Department of Biochemistry, Faculty of Life Sciences, Kebbi State University of Science and Technology Aliero, Kebbi State, Nigeria
  • Jude Nwaogu Department of Biochemistry, Faculty of Life Sciences, Kebbi State University of Science and Technology Aliero, Kebbi State, Nigeria
  • Ibrahim Sani Department of Biochemistry, Faculty of Life Sciences, Kebbi State University of Science and Technology Aliero, Kebbi State, Nigeria

DOI:

https://doi.org/10.25081/jmhe.2023.v9.8686

Keywords:

Diabetes mellitus, Piliostigma thonningii, Alpha-glucosidase, Hemoglobin glycosylation, Column chromatography

Abstract

Diabetes mellitus is a metabolic disease characterized by high blood sugar that profoundly affects human health and economy. Although there are a plethora of antidiabetic drugs in the market, the efficacy shown by some medicinal plants sometimes outperforms that of synthetic drugs while being more affordable and less toxic. This study was conducted to evaluate the anti-diabetic potential of column-chromatographic fractions of Piliostigma thonningii leaf. Phytochemical screening, column, and thin layer chromatography were respectively conducted following standard laboratory protocols. The in-vitro antidiabetic activity of the extracts was assessed using alpha-glucosidase and hemoglobin glycosylation inhibitory methods. A total of 175 column fractions (CF1-CF175) were eluted respectively. Thin layer chromatography protocols employed pooled a total of 11 (A-J) fractions. The result showed significant (P>0.05) inhibition of alpha-glucosidase in only fraction J2, which was significantly greater when compared to standard drug (Voglibose) and crude extract. Also, there was no significant hemoglobin glycosylation inhibitory effect in all fractions compared with crude extract and standard drugs (Voglibose). In conclusion, P. thonningii leaf exhibited alpha-glucosidase and hemoglobin glycosylation inhibitory potentials that further support its usage as a hypoglycemic agent.

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Published

25-12-2023

How to Cite

Ukwuani-Kwaja, A. N., Suru, I. M., Nwaogu, J., & Sani, I. (2023). In-Vitro antidiabetic potential of Piliostigma thonningii leaf column chromatographic fractions. Journal of Medicinal Herbs and Ethnomedicine, 9, 34–37. https://doi.org/10.25081/jmhe.2023.v9.8686

Issue

Volume 9, 2023

Section

Articles

Copyright (c) 2023 Angela Nnenna Ukwuani-Kwaja, Ibrahim Mohammad Suru, Jude Nwaogu, Ibrahim Sani

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This work is licensed under a Creative Commons Attribution 4.0 International License.