Phytochemical screening, antioxidant activity and toxicity of the orchids Prosthechea cochleata and Prosthechea livida-A preliminary study

  • Josué Nicanor Díaz Avilés Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Tuxpan Veracruz, Mexico https://orcid.org/0000-0003-4932-4674
  • José Luis Alanís Méndez Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Tuxpan Veracruz, Mexico https://orcid.org/0000-0002-2140-7139
  • Nahim Salgado Medrano Centro de investigación en Biotecnología (CEIB), Universidad Autónoma del Estado de Morelos, Cuernavaca Morelos, Mexico https://orcid.org/0000-0002-3911-0130
  • Alexandre Tochirico Cardoso Taketa Centro de investigación en Biotecnología (CEIB), Universidad Autónoma del Estado de Morelos, Cuernavaca Morelos, Mexico https://orcid.org/0000-0002-7055-0062
  • Ivette Alicia Chamorro Florescano Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Tuxpan Veracruz, Mexico https://orcid.org/0000-0003-3770-3394
  • Oswaldo Javier Enciso Díaz Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, Tuxpan Veracruz, Mexico

Abstract

Phytochemical composition, antioxidant, and toxic effects of two orchid (Prosthechea cochleata and Prosthechea livida) were examined in their different hexane and dichloromethane/methanol extracts of leaves, pseudobulbs, rhizome and roots.  Phytochemical screening reveals the presence of alkaloids, coumarins, flavonoids, saponins, steroids and terpenes except for anthracene derivatives in P. livida and tannins in both species. Results showed exhibited significant toxicity against brine shrimp with an LC50 values ranged from 3 to 54 µg/ml in 24 h.  Finally, the results of the antioxidant test in yeast (Saccharomyces cerevisiae) showed that CH2Cl2:MeOH extract of P. cochleata root extract presented the most efficient protective antioxidant percentage (%PA), with 20 µg/mL, displaying a cell viability of 68.58%. In P. livida case, rhizome extract presented the most efficient %PA, with 1000 µg/mL, with a cell viability of 65.82%.  The results evidenced the toxicity of  Prosthechea cochleata and Prosthechea livida and reveals potential antioxidative of extracts.

Keywords: Brine-Shrimp, LC50, medicinal orchids, plant extracts, Saccharomyces cerevisiae, toxicity

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How to Cite
Avilés, J. N. D., J. L. A. Méndez, N. S. Medrano, A. T. C. Taketa, I. A. C. Florescano, and O. J. E. Díaz. “Phytochemical Screening, Antioxidant Activity and Toxicity of the Orchids Prosthechea Cochleata and Prosthechea Livida-A Preliminary Study”. Journal of Medicinal Herbs and Ethnomedicine, Vol. 6, Dec. 2020, pp. 117-22, doi:https://doi.org/10.25081/jmhe.2020.v6.6431.
Section
Research Article