Studies on Antimicrobial Activities of Chaetomium atrobrunneum Ames against Selected Microorganisms

Abstract

The antibacterial and antifungal activities of Chaetomium atrobrunneum was investigated against Staphylococcus aureus (Gram +ve), Escherichia coli (Gram –ve) and Candida albicans using well diffusion and dilution method. The solvents used for extraction experiment was Ethylacetate and Dichloromethane and were removed in vacuo to yield viscous oils and paste which were made upto M₁D DCM extract-0.04g, M₁D EtOAc extract-0.03g, PDB EtOAc extract-0.04g, YPS EtOAc extract-0.025g dissolved in 10ml of DMSO each respectively. These were tested in varying volumes of 100-200µl/plate. Ampicillin and Itraconazole were used as references for bacteria and fungi . The solvent extracts of Chaetomium atrobrunneum showed higher antimicrobial inhibitory activity at 0.8mg/200µl of PDB EtOAc extract plate and the antioxidant activity test has been done with this PDB EtOAc extract. The extract which showed the higher antimicrobial activity is PDB-ethylacetate extract was taken for antioxidant study.