Callus induction from various explants of French bean (Phaseolus vulgaris L.)

Abstract

Callus cultures were studied in white seed induced mutant obtained from Phaseolus vulgarisL., cv. varunto help establish a suitable protocol for a prospective in vitro program. Callus cultures were initiated from the axillary leaves, axillary shoots, node, internode, and root segments.The initiation and growth of callus were evaluated on MS medium with 3% sucrose, 0.4% agar, 1.5 mg.l^-1BAP, and three levels of IAA. The highest callus relative growth was obtained on medium with 0.5 mg.l^-1IAA and 1.5 mg.l^-1BAP.